New Radiolabeled Exendin Analogues Show Reduced Renal Retention.


Journal

Molecular pharmaceutics
ISSN: 1543-8392
Titre abrégé: Mol Pharm
Pays: United States
ID NLM: 101197791

Informations de publication

Date de publication:
03 07 2023
Historique:
medline: 4 7 2023
pubmed: 2 6 2023
entrez: 2 6 2023
Statut: ppublish

Résumé

PET imaging of the glucagon-like peptide-1 receptor (GLP-1R) using radiolabeled exendin is a promising imaging method to detect insulinomas. However, high renal accumulation of radiolabeled exendin could hamper the detection of small insulinomas in proximity to the kidneys and limit its use as a radiotherapeutic agent. Here, we report two new exendin analogues for GLP-1R imaging and therapy, designed to reduce renal retention by incorporating a cleavable methionine-isoleucine (Met-Ile) linker. We examined the renal retention and insulinoma targeting properties of these new exendin analogues in a nude mouse model bearing subcutaneous GLP-1R-expressing insulinomas. NOTA or DOTA was conjugated via a methionine-isoleucine linker to the C-terminus of exendin-4 (NOTA-MI-exendin-4 or DOTA-MI-exendin-4). NOTA- and DOTA-exendin-4 without the linker were used as references. The affinity for GLP-1R was determined in a competitive binding assay using GLP-1R transfected cells. Biodistribution of [

Identifiants

pubmed: 37265006
doi: 10.1021/acs.molpharmaceut.3c00117
pmc: PMC10324399
doi:

Substances chimiques

Exenatide 9P1872D4OL
Gallium Radioisotopes 0
Isoleucine 04Y7590D77
Glucagon-Like Peptide-1 Receptor 0
Methionine AE28F7PNPL

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3519-3528

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Auteurs

Lieke Joosten (L)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Cathelijne Frielink (C)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Theodorus J P Jansen (TJP)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Daphne Lobeek (D)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Fritz Andreae (F)

Forschungs- und Entwicklungs GmbH, piCHEM, Parkring 3, 8074 Grambach, Austria.

Mark Konijnenberg (M)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Sandra Heskamp (S)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Martin Gotthardt (M)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

Maarten Brom (M)

Department of Medical Imaging, Nuclear Medicineof Medical Imaging, Radboud University Medical Center, 6525 GA Nijmegen, The Netherlands.

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Classifications MeSH