Thioredoxin TRXo1 is involved in ABA perception via PYR1 redox regulation.
Abscisic acid
Oligomerization
PYR1
Protein interaction
Redox regulation
Thioredoxin o1
Journal
Redox biology
ISSN: 2213-2317
Titre abrégé: Redox Biol
Pays: Netherlands
ID NLM: 101605639
Informations de publication
Date de publication:
07 2023
07 2023
Historique:
received:
21
03
2023
revised:
28
04
2023
accepted:
15
05
2023
medline:
7
6
2023
pubmed:
4
6
2023
entrez:
3
6
2023
Statut:
ppublish
Résumé
Abscisic acid (ABA) plays a fundamental role in plant growth and development processes such as seed germination, stomatal response or adaptation to stress, amongst others. Increases in the endogenous ABA content is recognized by specific receptors of the PYR/PYL/RCAR family that are coupled to a phosphorylation cascade targeting transcription factors and ion channels. Just like other receptors of the family, nuclear receptor PYR1 binds ABA and inhibits the activity of type 2C phosphatases (PP2Cs), thus avoiding the phosphatase-exerted inhibition on SnRK2 kinases, positive regulators which phosphorylate targets and trigger ABA signalling. Thioredoxins (TRXs) are key components of cellular redox homeostasis that regulate specific target proteins through a thiol-disulfide exchange, playing an essential role in redox homeostasis, cell survival, and growth. In higher plants, TRXs have been found in almost all cellular compartments, although its presence and role in nucleus has been less studied. In this work, affinity chromatography, Dot-blot, co-immunoprecipitation, and bimolecular fluorescence complementation assays allowed us to identify PYR1 as a new TRXo1 target in the nucleus. Studies on recombinant HisAtPYR1 oxidation-reduction with wild type and site-specific mutagenized forms showed that the receptor underwent redox regulation involving changes in the oligomeric state in which Cys
Identifiants
pubmed: 37269685
pii: S2213-2317(23)00151-9
doi: 10.1016/j.redox.2023.102750
pmc: PMC10244696
pii:
doi:
Substances chimiques
Abscisic Acid
72S9A8J5GW
Arabidopsis Proteins
0
Carrier Proteins
0
Phosphoric Monoester Hydrolases
EC 3.1.3.2
Thioredoxins
52500-60-4
Pyr1 protein, Arabidopsis
0
Membrane Transport Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102750Informations de copyright
Copyright © 2023 Consejo Superior de Investigaciones Científicas. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest None.
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