Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines.
Anticancer
Natural compound
Osteosarcoma
Rhabdomyosarcoma
Violacein
Journal
Journal of cancer research and clinical oncology
ISSN: 1432-1335
Titre abrégé: J Cancer Res Clin Oncol
Pays: Germany
ID NLM: 7902060
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
28
03
2023
accepted:
23
05
2023
medline:
31
8
2023
pubmed:
4
6
2023
entrez:
4
6
2023
Statut:
ppublish
Résumé
Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines. The toxicity of violacein was assessed in vitro and in vivo, using MTT assay and FET test. The effect of violacein on cell migration was monitored by wound healing assay, cell death by flow cytometry, uptake of violacein by fluorescence microscopy, generation of reactive oxygen species (ROS) by DCFH-DA assay and lipid peroxidation by TBARS assay. Violacein IC Our study provided further evidence that reinforces the potential of violacein as an anticancer agent and candidate to consider for improvement of the effectiveness of traditional OS and RMS therapies.
Identifiants
pubmed: 37270734
doi: 10.1007/s00432-023-04930-9
pii: 10.1007/s00432-023-04930-9
doi:
Substances chimiques
violacein
QJH0DSQ3SG
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10975-10987Subventions
Organisme : Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja
ID : 173008, 451-03-68/2020-14/200042, 451-03-9/2021-14/200042, 451-03-68/2022-14/200042
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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