Inhibition of Infectious HIV-1 Production by Rerouting the Cellular Furin Inhibitor Serpin B8.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
29 06 2023
Historique:
medline: 3 7 2023
pubmed: 5 6 2023
entrez: 5 6 2023
Statut: ppublish

Résumé

Serpins are a superfamily of proteins that regulate a variety of physiological processes by irreversibly inhibiting the enzymatic activity of different serine proteases. For example, Serpin Family B Member 8 (Serpin B8, also known as PI8 and CAP2) binds to and inhibits the proprotein convertase furin. Like many other viral pathogens, human immunodeficiency virus type 1 (HIV-1) exploits furin for the proteolytic activation of its envelope glycoprotein (Env). Since the furin inhibitor Serpin B8 is expressed in primary target cells of HIV-1 and induced under inflammatory conditions, we hypothesized that it might interfere with HIV-1 Env maturation and decrease infectivity of newly produced virions. Indeed, recombinant Serpin B8 reduced furin-mediated cleavage of an HIV-1 Env reporter substrate

Identifiants

pubmed: 37272794
doi: 10.1128/jvi.00294-23
pmc: PMC10308892
doi:

Substances chimiques

env Gene Products, Human Immunodeficiency Virus 0
Furin EC 3.4.21.75
Serpins 0
SERPINB8 protein, human 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0029423

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Moritz Petersen (M)

Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.

Rishikesh Lotke (R)

Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.

Kristina Hopfensperger (K)

Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.

Sabina Victoria (S)

Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.

Isabell Haußmann (I)

Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.

Timo Burster (T)

School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan.

Hanna-Mari Baldauf (HM)

Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU Munich, Munich, Germany.

Daniel Sauter (D)

Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital Tübingen, Tübingen, Germany.

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Classifications MeSH