Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 06 2023
05 06 2023
Historique:
received:
22
01
2023
accepted:
28
05
2023
medline:
7
6
2023
pubmed:
6
6
2023
entrez:
5
6
2023
Statut:
epublish
Résumé
Three polysaccharide matrices (κ-Carrageenan (Carr), gellan gum, and agar) were grafted via glutaraldehyde (GA) and pea protein (PP). The grafted matrices covalently immobilized β-D-galactosidase (β-GL). Nonetheless, grafted Carr acquired the topmost amount of immobilized β-GL (iβ-GL). Thus, its grafting process was honed via Box-Behnken design and was further characterized via FTIR, EDX, and SEM. The optimal GA-PP-Carr grafting comprised processing Carr beads with 10% PP dispersion of pH 1 and 25% GA solution. The optimal GA-PP-Carr beads acquired 11.44 Ug
Identifiants
pubmed: 37277367
doi: 10.1038/s41598-023-36045-z
pii: 10.1038/s41598-023-36045-z
pmc: PMC10241775
doi:
Substances chimiques
Enzymes, Immobilized
0
Glutaral
T3C89M417N
Pea Proteins
0
Lactose
J2B2A4N98G
Carrageenan
9000-07-1
beta-Galactosidase
EC 3.2.1.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9105Informations de copyright
© 2023. The Author(s).
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