iAstrocytes do not restrain T cell proliferation in vitro.


Journal

BMC neuroscience
ISSN: 1471-2202
Titre abrégé: BMC Neurosci
Pays: England
ID NLM: 100966986

Informations de publication

Date de publication:
07 06 2023
Historique:
received: 18 01 2023
accepted: 26 05 2023
medline: 9 6 2023
pubmed: 8 6 2023
entrez: 7 6 2023
Statut: epublish

Résumé

The cross-talk between T cells and astrocytes occurring under physiological and, even more, neuroinflammatory conditions may profoundly impact the generation of adaptive immune responses in the nervous tissue. In this study, we used a standardized in vitro co-culture assay to investigate the immunomodulatory properties of astrocytes differing for age, sex, and species. Mouse neonatal astrocytes enhanced T cell vitality but suppressed T lymphocyte proliferation in response to mitogenic stimuli or myelin antigens, regardless of the Th1, Th2 or Th17 T cell phenotype. Studies comparing glia cells from adult and neonatal animals showed that adult astrocytes were more efficient in inhibiting T lymphocyte activation than neonatal astrocytes, regardless of their sex. Differently from primary cultures, mouse and human astrocytes derived from reprogrammed fibroblasts did not interfere with T cell proliferation. Overall, we describe a standardized astrocyte-T cell interaction in vitro assay and demonstrate that primary astrocytes and iAstrocytes may differ in modulating T cell function.

Identifiants

pubmed: 37286983
doi: 10.1186/s12868-023-00806-3
pii: 10.1186/s12868-023-00806-3
pmc: PMC10245549
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

33

Informations de copyright

© 2023. The Author(s).

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Auteurs

Emanuela Colombo (E)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Anthea De Angelis (A)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Claudia Bassani (C)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Francesca Ruffini (F)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Linda Ottoboni (L)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Livia Garzetti (L)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Annamaria Finardi (A)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Gianvito Martino (G)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.
Vita-Salute San Raffaele University, Milan, Italy.

Roberto Furlan (R)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy.

Cinthia Farina (C)

Institute of Experimental Neurology (INSpe), Division of Neuroscience, IRCCS San Raffaele Hospital, Milan, Italy. farina.cinthia@hsr.it.

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