Genome-scale targeted mutagenesis in
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
received:
03
01
2023
accepted:
19
04
2023
medline:
19
6
2023
pubmed:
9
6
2023
entrez:
8
6
2023
Statut:
ppublish
Résumé
The recently constructed mutant libraries of diploid crops by the CRISPR-Cas9 system have provided abundant resources for functional genomics and crop breeding. However, because of the genome complexity, it is a big challenge to accomplish large-scale targeted mutagenesis in polyploid plants. Here, we demonstrate the feasibility of using a pooled CRISPR library to achieve genome-scale targeted editing in an allotetraploid crop of
Identifiants
pubmed: 37290935
pii: gr.277650.123
doi: 10.1101/gr.277650.123
pmc: PMC10317123
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
798-809Informations de copyright
© 2023 He et al.; Published by Cold Spring Harbor Laboratory Press.
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