The structure of Arabidopsis phytochrome A reveals topological and functional diversification among the plant photoreceptor isoforms.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
07 2023
07 2023
Historique:
received:
14
12
2022
accepted:
10
05
2023
medline:
21
7
2023
pubmed:
9
6
2023
entrez:
8
6
2023
Statut:
ppublish
Résumé
Plants employ a divergent cohort of phytochrome (Phy) photoreceptors to govern many aspects of morphogenesis through reversible photointerconversion between inactive Pr and active Pfr conformers. The two most influential are PhyA whose retention of Pfr enables sensation of dim light, while the relative instability of Pfr for PhyB makes it better suited for detecting full sun and temperature. To better understand these contrasts, we solved, by cryo-electron microscopy, the three-dimensional structure of full-length PhyA as Pr. Like PhyB, PhyA dimerizes through head-to-head assembly of its C-terminal histidine kinase-related domains (HKRDs), while the remainder assembles as a head-to-tail light-responsive platform. Whereas the platform and HKRDs associate asymmetrically in PhyB dimers, these lopsided connections are absent in PhyA. Analysis of truncation and site-directed mutants revealed that this decoupling and altered platform assembly have functional consequences for Pfr stability of PhyA and highlights how plant Phy structural diversification has extended light and temperature perception.
Identifiants
pubmed: 37291396
doi: 10.1038/s41477-023-01435-8
pii: 10.1038/s41477-023-01435-8
pmc: PMC10546791
mid: NIHMS1928402
doi:
Substances chimiques
Arabidopsis Proteins
0
Photoreceptors, Plant
0
Phytochrome
11121-56-5
Phytochrome A
0
Phytochrome B
136250-22-1
Protein Isoforms
0
PHYA protein, Arabidopsis
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1116-1129Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM127892
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131754
Pays : United States
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Limited.
Références
Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10179-84
pubmed: 24982198
Proc Natl Acad Sci U S A. 2020 Jan 7;117(1):300-307
pubmed: 31852825
J Struct Biol. 2015 Nov;192(2):216-21
pubmed: 26278980
Cell. 1997 Dec 12;91(6):713-6
pubmed: 9413979
Acta Crystallogr D Struct Biol. 2017 Oct 01;73(Pt 10):793-803
pubmed: 28994408
J Struct Biol. 2021 Jun;213(2):107702
pubmed: 33582281
Nat Genet. 2001 Dec;29(4):441-6
pubmed: 11726931
J Biol Chem. 2016 Sep 23;291(39):20674-91
pubmed: 27466363
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Nature. 2003 Jul 31;424(6948):571-4
pubmed: 12891362
Protein Sci. 2018 Jan;27(1):293-315
pubmed: 29067766
Nature. 2001 Dec 13;414(6865):776-9
pubmed: 11742406
Crit Rev Biochem Mol Biol. 2011 Feb;46(1):67-88
pubmed: 21250783
Science. 2014 Jun 6;344(6188):1160-1164
pubmed: 24904166
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Nat Commun. 2021 Jul 20;12(1):4394
pubmed: 34285211
New Phytol. 2020 Mar;225(6):2283-2300
pubmed: 31595505
Structure. 2013 Jul 2;21(7):1127-36
pubmed: 23746806
Genome Biol. 2002 Sep 25;3(10):REVIEWS3013
pubmed: 12372152
Plant Physiol. 2018 Feb;176(2):1025-1038
pubmed: 29138351
Proc Natl Acad Sci U S A. 2021 Jun 1;118(22):
pubmed: 34039713
Commun Biol. 2019 Dec 2;2:448
pubmed: 31815202
Nat Plants. 2020 May;6(5):581-588
pubmed: 32366982
Plant Cell. 2014 Dec;26(12):4568-83
pubmed: 25480369
Nat Commun. 2019 Nov 19;10(1):5219
pubmed: 31745087
Sci Adv. 2017 Mar 03;3(3):e1602498
pubmed: 28275738
Plant Cell. 2012 Jul;24(7):2949-62
pubmed: 22843485
Proc Natl Acad Sci U S A. 2019 Apr 23;116(17):8603-8608
pubmed: 30948632
Science. 1997 Sep 5;277(5331):1505-8
pubmed: 9278513
Proc Natl Acad Sci U S A. 2020 Jan 21;117(3):1496-1503
pubmed: 31896580
Structure. 2016 Mar 1;24(3):448-57
pubmed: 26853942
IUCrJ. 2018 Aug 29;5(Pt 5):619-634
pubmed: 30224965
Commun Biol. 2021 Jul 15;4(1):874
pubmed: 34267316
Cell. 2009 Oct 16;139(2):325-36
pubmed: 19800110
Nat Commun. 2015 Jul 28;6:7852
pubmed: 26215968
Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14715-20
pubmed: 18799746
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
J Biol Chem. 2014 Aug 29;289(35):24573-87
pubmed: 25006244
J Biol Chem. 2018 Jun 8;293(23):9078-9089
pubmed: 29695503
PLoS Genet. 2009 Jan;5(1):e1000352
pubmed: 19165330
Nature. 2022 Apr;604(7904):127-133
pubmed: 35355010
Nature. 2014 May 8;509(7499):245-248
pubmed: 24776794
Plant Cell. 2004 Aug;16(8):2104-16
pubmed: 15273294
Science. 2016 Nov 18;354(6314):886-889
pubmed: 27789797
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13976-81
pubmed: 9811911
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14709-14
pubmed: 18799745
Structure. 2015 Jul 7;23(7):1179-89
pubmed: 26095026
Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):8169-74
pubmed: 10869441
Science. 2016 Nov 18;354(6314):897-900
pubmed: 27789798
J Biol Chem. 2008 May 2;283(18):12212-26
pubmed: 18192276
Nature. 2005 Nov 17;438(7066):325-31
pubmed: 16292304
J Exp Bot. 2010;61(1):11-24
pubmed: 19815685
Structure. 2021 Feb 4;29(2):151-160.e3
pubmed: 32916102
Nat Commun. 2016 May 13;7:11545
pubmed: 27173885
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
Acta Crystallogr D Struct Biol. 2017 Jun 1;73(Pt 6):496-502
pubmed: 28580911
PLoS Biol. 2013;11(2):e1001493
pubmed: 23468592
J Biol Chem. 2013 Dec 13;288(50):35714-25
pubmed: 24174528
Structure. 2021 Jul 1;29(7):743-754.e4
pubmed: 33756101
Protein Sci. 2018 Jan;27(1):14-25
pubmed: 28710774
Sci Rep. 2017 Oct 20;7(1):13648
pubmed: 29057954