A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
09 06 2023
Historique:
received: 05 10 2022
accepted: 27 04 2023
medline: 12 6 2023
pubmed: 10 6 2023
entrez: 9 6 2023
Statut: epublish

Résumé

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) protects against cardiovascular disease by metabolising the risk factor asymmetric dimethylarginine (ADMA). However, the question whether the second DDAH isoform, DDAH2, directly metabolises ADMA has remained unanswered. Consequently, it is still unclear if DDAH2 may be a potential target for ADMA-lowering therapies or if drug development efforts should focus on DDAH2's known physiological functions in mitochondrial fission, angiogenesis, vascular remodelling, insulin secretion, and immune responses. Here, an international consortium of research groups set out to address this question using in silico, in vitro, cell culture, and murine models. The findings uniformly demonstrate that DDAH2 is incapable of metabolising ADMA, thus resolving a 20-year controversy and providing a starting point for the investigation of alternative, ADMA-independent functions of DDAH2.

Identifiants

pubmed: 37296100
doi: 10.1038/s41467-023-38467-9
pii: 10.1038/s41467-023-38467-9
pmc: PMC10256801
doi:

Substances chimiques

dimethylargininase EC 3.5.3.18
Amidohydrolases EC 3.5.-
Arginine 94ZLA3W45F
Nitric Oxide 31C4KY9ESH

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3392

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL140411
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL148339
Pays : United States

Informations de copyright

© 2023. The Author(s).

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Auteurs

Vinitha N Ragavan (VN)

Department of Internal Medicine III, Technische Universität Dresden, Dresden, Germany.
Department of Clinical Pharmacology, College of Medicine and Public Health, Flinders University and Flinders Medical Centre, Bedford Park, Adelaide, SA, Australia.

Pramod C Nair (PC)

Department of Clinical Pharmacology, College of Medicine and Public Health, Flinders University and Flinders Medical Centre, Bedford Park, Adelaide, SA, Australia.
Flinders Health and Medical Research Institute (FHMRI), College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
Cancer Program, South Australian Health and Medical Research Institute (SAHMRI), University of Adelaide, Adelaide, SA, Australia.
Discipline of Medicine, Adelaide Medical School, University of Adelaide, Adelaide, SA, Australia.

Natalia Jarzebska (N)

Department of Internal Medicine III, Technische Universität Dresden, Dresden, Germany.

Ramcharan Singh Angom (RS)

Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Jacksonville, FL, USA.

Luana Ruta (L)

Department of Pharmaceutical Sciences, University of Perugia, via del Liceo 1, Perugia, Italy.

Elisa Bianconi (E)

Department of Pharmaceutical Sciences, University of Perugia, via del Liceo 1, Perugia, Italy.

Silvia Grottelli (S)

Department of Medicine and Surgery, University of Perugia, P.le L. Sevari 1, Perugia, Italy.

Natalia D Tararova (ND)

DAPCEL, Inc., Cleveland, OH, USA.

Daniel Ryazanskiy (D)

DAPCEL, Inc., Cleveland, OH, USA.

Steven R Lentz (SR)

Department of Internal Medicine, The University of Iowa Carver College of Medicine, Iowa City, IA, USA.

Sara Tommasi (S)

Department of Clinical Pharmacology, College of Medicine and Public Health, Flinders University and Flinders Medical Centre, Bedford Park, Adelaide, SA, Australia.

Jens Martens-Lobenhoffer (J)

Institute of Clinical Pharmacology, Otto von Guericke University, Magdeburg, Germany.

Toshiko Suzuki-Yamamoto (T)

Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Okayama, Japan.

Masumi Kimoto (M)

Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Okayama, Japan.

Elena Rubets (E)

Department of Internal Medicine III, Technische Universität Dresden, Dresden, Germany.

Sarah Chau (S)

Department of Cardiovascular Medicine, Mayo Clinic College of Medicine and Science, Rochester, NY, USA.

Yingjie Chen (Y)

Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS, USA.

Xinli Hu (X)

Institute of Molecular Medicine, Beijing University, Beijing, China.

Nadine Bernhardt (N)

Department of Psychiatry and Psychotherapy, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Peter M Spieth (PM)

Department of Anesthesiology and Critical Care Medicine, University Hospital Dresden, Technische Universität Dresden, Dresden, Germany.

Norbert Weiss (N)

Department of Internal Medicine III, Technische Universität Dresden, Dresden, Germany.

Stefan R Bornstein (SR)

Department of Internal Medicine III, Technische Universität Dresden, Dresden, Germany.
School of Cardiovascular and Metabolic Medicine and Sciences, Faculty of Life Sciences & Medicine, King's College London, London, UK.

Debabrata Mukhopadhyay (D)

Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine and Science, Jacksonville, FL, USA.

Stefanie M Bode-Böger (SM)

Institute of Clinical Pharmacology, Otto von Guericke University, Magdeburg, Germany.

Renke Maas (R)

Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
FAU New - Research Center for New Bioactive Compounds, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.

Ying Wang (Y)

Department of Cardiovascular Medicine, Mayo Clinic College of Medicine and Science, Rochester, NY, USA.

Antonio Macchiarulo (A)

Department of Pharmaceutical Sciences, University of Perugia, via del Liceo 1, Perugia, Italy.

Arduino A Mangoni (AA)

Department of Clinical Pharmacology, College of Medicine and Public Health, Flinders University and Flinders Medical Centre, Bedford Park, Adelaide, SA, Australia.

Barbara Cellini (B)

Department of Medicine and Surgery, University of Perugia, P.le L. Sevari 1, Perugia, Italy.

Roman N Rodionov (RN)

Department of Internal Medicine III, Technische Universität Dresden, Dresden, Germany. Roman.Rodionov@uniklinikum-dresden.de.
College of Medicine and Public Health, Flinders University and Flinders Medical Center, Adelaide, SA, Australia. Roman.Rodionov@uniklinikum-dresden.de.

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