Dynamic Tumor Perfusion and Real-Time Monitoring in a Multiplexed 3D Printed Microdevice.
3D printing
Cancer
Microfluidics
Monolithic
Perfusion
Spheroid
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
12
6
2023
pubmed:
10
6
2023
entrez:
10
6
2023
Statut:
ppublish
Résumé
Stereolithography based additive manufacturing ("3D printing") has become a useful tool for the development of novel microfluidic in vitro platforms. This method of manufacturing can reduce production time while allowing for rapid design iteration and complex monolithic structures. The platform described in this chapter has been designed for the capture and evaluation of cancer spheroids in perfusion. Spheroids are created in 3D Petri dishes, stained, and loaded into these 3D printed devices and imaged over time under flow conditions. This design allows for active perfusion into complex 3D cellular constructs resulting in longer viability while providing results which better mimic in vivo conditions compared to traditional monolayer static culture.
Identifiants
pubmed: 37300624
doi: 10.1007/978-1-0716-3271-0_20
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
287-304Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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