Silymarin mediated osmotic responses and damage in HepG2 cell suspensions and monolayers.
Cell suspensions
Cryopreservation
HepG2 cells
Hepatoprotective
Monolayers
Osmotic damage
Time dependent
silymarin
Journal
Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
18
04
2023
revised:
19
05
2023
accepted:
07
06
2023
medline:
29
8
2023
pubmed:
11
6
2023
entrez:
10
6
2023
Statut:
ppublish
Résumé
Maintenance of cells within a volume range compatible with their functional integrity is a critical determinant of cell survival after cryopreservation, and quantifying this osmotically induced damage is a part of the rational design of improved cryopreservation protocols. The degree that cells tolerate osmotic stress significantly impacts applicable cryoprotocols, but there has been little research on the time dependence of this osmotic stress. Additionally, the flavonoid silymarin has been shown to be hepatoprotective. Therefore, here we test the hypotheses that osmotic damage is time-dependent and that flavonoid inclusion reduces osmotic damage. In our first experiment, cells were exposed to a series of anisosmotic solutions of graded hypo- and hypertonicity for 10-40 min, resulting in a conclusion that osmotically induced damage is time dependent. In the next experiment, adherent cells preincubated with silymarin at the concentration of 10
Identifiants
pubmed: 37301358
pii: S0011-2240(23)00051-2
doi: 10.1016/j.cryobiol.2023.104552
pii:
doi:
Substances chimiques
Silymarin
0
Suspensions
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104552Subventions
Organisme : CIHR
ID : 425741
Pays : Canada
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.