Allosteric activation of cell wall synthesis during bacterial growth.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 06 2023
10 06 2023
Historique:
received:
18
01
2023
accepted:
25
05
2023
medline:
12
6
2023
pubmed:
11
6
2023
entrez:
10
6
2023
Statut:
epublish
Résumé
The peptidoglycan (PG) cell wall protects bacteria against osmotic lysis and determines cell shape, making this structure a key antibiotic target. Peptidoglycan is a polymer of glycan chains connected by peptide crosslinks, and its synthesis requires precise spatiotemporal coordination between glycan polymerization and crosslinking. However, the molecular mechanism by which these reactions are initiated and coupled is unclear. Here we use single-molecule FRET and cryo-EM to show that an essential PG synthase (RodA-PBP2) responsible for bacterial elongation undergoes dynamic exchange between closed and open states. Structural opening couples the activation of polymerization and crosslinking and is essential in vivo. Given the high conservation of this family of synthases, the opening motion that we uncovered likely represents a conserved regulatory mechanism that controls the activation of PG synthesis during other cellular processes, including cell division.
Identifiants
pubmed: 37301887
doi: 10.1038/s41467-023-39037-9
pii: 10.1038/s41467-023-39037-9
pmc: PMC10257715
doi:
Substances chimiques
Bacterial Proteins
0
Penicillin-Binding Proteins
0
Peptidoglycan
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3439Subventions
Organisme : NIAID NIH HHS
ID : R01 AI153358
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI083365
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI158028
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM114065
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI148752
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2023. The Author(s).
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