Identification and characterization of an abundant lipoprotein from Methylacidiphilum fumariolicum SolV.
Acidophile
Cell wall
Lipoprotein
Methanotroph
Methylacidiphilum
Peptidoglycan
Verrucomicrobiota
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
12 Jun 2023
12 Jun 2023
Historique:
received:
05
04
2023
accepted:
30
05
2023
revised:
17
05
2023
medline:
14
6
2023
pubmed:
12
6
2023
entrez:
12
6
2023
Statut:
epublish
Résumé
Bacterial lipoproteins are characterized by the presence of a conserved N-terminal lipid-modified cysteine residue that allows the hydrophilic protein to anchor into bacterial cell membranes. These lipoproteins play essential roles in a wide variety of physiological processes. Based on transcriptome analysis of the verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV, we identified a highly expressed lipoprotein, WP_009060351 (139 amino acids), in its genome. The first 86 amino acids are specific for the methanotrophic genera Methylacidiphilum and Methylacidmicrobium, while the last 53 amino acids are present only in lipoproteins of members from the phylum Verrucomicrobiota (Hedlund). Heterologous expression of WP_009060351 in Escherichia coli revealed a 25-kDa dimeric protein and a 60-kDa tetrameric protein. Immunoblotting showed that WP_009060351 was present in the total membrane protein and peptidoglycan fractions of M. fumariolicum SolV. The results suggest an involvement of lipoprotein WP_009060351 in the linkage between the outer membrane and the peptidoglycan.
Identifiants
pubmed: 37306788
doi: 10.1007/s00203-023-03603-y
pii: 10.1007/s00203-023-03603-y
pmc: PMC10260701
doi:
Substances chimiques
Peptidoglycan
0
Lipoproteins
0
Amino Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
261Subventions
Organisme : Chinese Scholarship Council
ID : CQL: 201804910641
Organisme : European Research Council Advanced Grant
ID : VOLCANO 669371
Organisme : European Research Council Advanced Grant
ID : VOLCANO 669371
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : NWO VI.Vidi.192.001
Informations de copyright
© 2023. The Author(s).
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