Cell-specific RNA profiling reveals host genes expressed in Arabidopsis cells haustoriated by downy mildew.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
31 08 2023
31 08 2023
Historique:
received:
17
01
2023
accepted:
15
05
2023
medline:
1
9
2023
pubmed:
12
6
2023
entrez:
12
6
2023
Statut:
ppublish
Résumé
The downy mildew oomycete Hyaloperonospora arabidopsidis, an obligate filamentous pathogen, infects Arabidopsis (Arabidopsis thaliana) by forming structures called haustoria inside host cells. Previous transcriptome analyses have revealed that host genes are specifically induced during infection; however, RNA profiling from whole-infected tissues may fail to capture key transcriptional events occurring exclusively in haustoriated host cells, where the pathogen injects virulence effectors to modulate host immunity. To determine interactions between Arabidopsis and H. arabidopsidis at the cellular level, we devised a translating ribosome affinity purification system using 2 high-affinity binding proteins, colicin E9 and Im9 (immunity protein of colicin E9), applicable to pathogen-responsive promoters, thus enabling haustoriated cell-specific RNA profiling. Among the host genes specifically expressed in H. arabidopsidis-haustoriated cells, we found genes that promote either susceptibility or resistance to the pathogen, providing insights into the Arabidopsis-downy mildew interaction. We propose that our protocol for profiling cell-specific transcripts will apply to several stimulus-specific contexts and other plant-pathogen interactions.
Identifiants
pubmed: 37307565
pii: 7194663
doi: 10.1093/plphys/kiad326
pmc: PMC10469357
doi:
Substances chimiques
RNA
63231-63-0
Colicins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
259-270Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.
Déclaration de conflit d'intérêts
Conflict of interest statement. None declared.
Références
Essays Biochem. 2022 Sep 30;66(5):647-656
pubmed: 35698792
Plant Physiol. 2017 Nov;175(3):1082-1093
pubmed: 28899963
Biochem J. 2020 Aug 28;477(16):3019-3032
pubmed: 32744327
Plant J. 2012 Jan;69(2):252-65
pubmed: 21914011
Plant Physiol. 2012 Sep;160(1):365-78
pubmed: 22822212
Biochemistry. 1995 Oct 24;34(42):13743-50
pubmed: 7577966
Mol Plant. 2008 May;1(3):482-95
pubmed: 19825555
ACS Synth Biol. 2014 Nov 21;3(11):839-43
pubmed: 24933124
Science. 2009 Apr 3;324(5923):89-91
pubmed: 19342588
Plant J. 2010 Dec;64(6):912-23
pubmed: 21143673
Plant Cell. 2006 May;18(5):1310-26
pubmed: 16603654
Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18843-8
pubmed: 19843695
Plant J. 2012 Jul;71(2):194-204
pubmed: 22353039
Plant Physiol Biochem. 2016 Oct;107:273-287
pubmed: 27337039
Methods Mol Biol. 2009;553:109-26
pubmed: 19588103
Nat Protoc. 2014;9(6):1282-91
pubmed: 24810037
Plant J. 2013 Feb;73(3):469-82
pubmed: 23062058
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Plant Cell. 2008 May;20(5):1390-406
pubmed: 18515503
Front Plant Sci. 2018 Jul 10;9:986
pubmed: 30042779
Plant J. 2022 Jul;111(1):304-315
pubmed: 35436375
Nat Commun. 2015 Jul 23;6:7658
pubmed: 26203923
New Phytol. 2018 Oct;220(1):232-248
pubmed: 30156022
BMC Genomics. 2014 May 06;15:341
pubmed: 24884414
Front Plant Sci. 2020 Nov 06;11:603693
pubmed: 33240308
Cell Host Microbe. 2021 Feb 10;29(2):299-310.e7
pubmed: 33378688
Genome Biol. 2004;5(10):R80
pubmed: 15461798
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Plant J. 2008 Jun;54(5):785-93
pubmed: 18248595
PLoS One. 2008;3(11):e3647
pubmed: 18985154
PLoS Biol. 2013 Dec;11(12):e1001732
pubmed: 24339748
Annu Rev Phytopathol. 2014;52:551-81
pubmed: 25001453
Plant Cell. 2017 Jan;29(1):20-38
pubmed: 28011690
Nucleic Acids Res. 2019 Jan 8;47(D1):D419-D426
pubmed: 30407594
Annu Rev Phytopathol. 2010;48:329-45
pubmed: 19400636
PLoS Pathog. 2014 Oct 16;10(10):e1004443
pubmed: 25329884