Ethanol inhibits dopamine uptake via organic cation transporter 3: Implications for ethanol and cocaine co-abuse.


Journal

Molecular psychiatry
ISSN: 1476-5578
Titre abrégé: Mol Psychiatry
Pays: England
ID NLM: 9607835

Informations de publication

Date de publication:
Jul 2023
Historique:
received: 05 10 2022
accepted: 29 03 2023
revised: 20 12 2022
medline: 1 11 2023
pubmed: 13 6 2023
entrez: 12 6 2023
Statut: ppublish

Résumé

Concurrent cocaine and alcohol use is among the most frequent drug combination, and among the most dangerous in terms of deleterious outcomes. Cocaine increases extracellular monoamines by blocking dopamine (DA), norepinephrine (NE) and serotonin (5-HT) transporters (DAT, NET and SERT, respectively). Likewise, ethanol also increases extracellular monoamines, however evidence suggests that ethanol does so independently of DAT, NET and SERT. Organic cation transporter 3 (OCT3) is an emergent key player in the regulation of monoamine signaling. Using a battery of in vitro, in vivo electrochemical, and behavioral approaches, as well as wild-type and constitutive OCT3 knockout mice, we show that ethanol's actions to inhibit monoamine uptake are dependent on OCT3. These findings provide a novel mechanistic basis whereby ethanol enhances the neurochemical and behavioral effects of cocaine and encourage further research into OCT3 as a target for therapeutic intervention in the treatment of ethanol and ethanol/cocaine use disorders.

Identifiants

pubmed: 37308680
doi: 10.1038/s41380-023-02064-5
pii: 10.1038/s41380-023-02064-5
pmc: PMC10615754
doi:

Substances chimiques

Dopamine VTD58H1Z2X
Ethanol 3K9958V90M
Carrier Proteins 0
Cocaine I5Y540LHVR
Serotonin 333DO1RDJY
Cations 0
Dopamine Plasma Membrane Transport Proteins 0
Serotonin Plasma Membrane Transport Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2934-2945

Subventions

Organisme : NIMH NIH HHS
ID : R01 MH093320
Pays : United States
Organisme : NIDA NIH HHS
ID : R21 DA046044
Pays : United States
Organisme : NIDA NIH HHS
ID : T32 DA031115
Pays : United States
Organisme : NIDA NIH HHS
ID : U18 DA052527
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH093320
Pays : United States

Informations de copyright

© 2023. The Author(s).

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Auteurs

N J Clauss (NJ)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

F P Mayer (FP)

Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.
Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DK-2200, Denmark.

W A Owens (WA)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

M Vitela (M)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

K M Clarke (KM)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

M A Bowman (MA)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

R E Horton (RE)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

D Gründemann (D)

Department of Pharmacology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931, Cologne, Germany.

D Schmid (D)

Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.

M Holy (M)

Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.

G G Gould (GG)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

W Koek (W)

Department of Cell Systems and Anatomy, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA.

H H Sitte (HH)

Center for Physiology and Pharmacology, Medical University of Vienna, 1090, Vienna, Austria.
Center for Addiction Research and Science, Medical University Vienna, Waehringerstrasse 13 A, 1090, Vienna, Austria.

L C Daws (LC)

Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA. daws@uthscsa.edu.
Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, TX, 78229, USA. daws@uthscsa.edu.

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