Global translational control by the transcriptional repressor TrcR in the filamentous cyanobacterium Anabaena sp. PCC 7120.

Bacterial Proteins / metabolism Anabaena / genetics Cyanobacteria / genetics Transcription Factors / metabolism Operon RNA, Transfer / metabolism

Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
15 06 2023
Historique:
received: 03 02 2023
accepted: 02 06 2023
medline: 19 6 2023
pubmed: 16 6 2023
entrez: 15 6 2023
Statut: epublish

Résumé

Transcriptional and translational regulations are important mechanisms for cell adaptation to environmental conditions. In addition to house-keeping tRNAs, the genome of the filamentous cyanobacterium Anabaena sp. strain PCC 7120 (Anabaena) has a long tRNA operon (trn operon) consisting of 26 genes present on a megaplasmid. The trn operon is repressed under standard culture conditions, but is activated under translational stress in the presence of antibiotics targeting translation. Using the toxic amino acid analog β-N-methylamino-L-alanine (BMAA) as a tool, we isolated and characterized several BMAA-resistance mutants from Anabaena, and identified one gene of unknown function, all0854, named as trcR, encoding a transcription factor belonging to the ribbon-helix-helix (RHH) family. We provide evidence that TrcR represses the expression of the trn operon and is thus the missing link between the trn operon and translational stress response. TrcR represses the expression of several other genes involved in translational control, and is required for maintaining translational fidelity. TrcR, as well as its binding sites, are highly conserved in cyanobacteria, and its functions represent an important mechanism for the coupling of the transcriptional and translational regulations in cyanobacteria.

Identifiants

DOI: 10.1038/s42003-023-05012-9 PMID: 37322092 PMC: PMC10272220
pubmed: 37322092
doi: 10.1038/s42003-023-05012-9
pii: 10.1038/s42003-023-05012-9
pmc: PMC10272220
doi:

Substances chimiques

Bacterial Proteins 0
Transcription Factors 0
RNA, Transfer 9014-25-9

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

643

Informations de copyright

© 2023. The Author(s).

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Auteurs

Zi-Qian Wang (ZQ)

State Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, People's Republic of China. wangziqian@im.ac.cn.
University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. wangziqian@im.ac.cn.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. wangziqian@im.ac.cn.
ORCID: 0000-0003-3715-1593

Yiling Yang (Y)

State Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, People's Republic of China.
ORCID: 0000-0002-2882-0743

Ju-Yuan Zhang (JY)

State Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, People's Republic of China.

Xiaoli Zeng (X)

State Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, People's Republic of China.

Cheng-Cai Zhang (CC)

State Key Laboratory of Freshwater Ecology and Biotechnology and Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei, 430072, People's Republic of China. cczhang@ihb.ac.cn.
Institute AMU-WUT, Aix-Marseille Université and Wuhan University of Technology, Wuhan, Hubei, People's Republic of China. cczhang@ihb.ac.cn.
ORCID: 0000-0002-8194-2889

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Global translational control by the...
questionsmedicales.fr › Bactéries › Bactéries fixatrices d'azote › Anabaena › Global translational control by the...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries à Gram négatif photosynthétiques › Cyanobactéries › Global translational control by the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Global translational control by the...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Opéron › Global translational control by the...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › Global translational control by the...