Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4
Annexin A1
DFNB4
Hearing loss
Pendred syndrome
Single-cell RNA sequencing
Slc26a4
Spindle cells
Stria vascularis cell
Journal
BMC medical genomics
ISSN: 1755-8794
Titre abrégé: BMC Med Genomics
Pays: England
ID NLM: 101319628
Informations de publication
Date de publication:
15 06 2023
15 06 2023
Historique:
received:
20
01
2023
accepted:
15
05
2023
medline:
19
6
2023
pubmed:
16
6
2023
entrez:
15
6
2023
Statut:
epublish
Résumé
The primary pathological alterations of Pendred syndrome are endolymphatic pH acidification and luminal enlargement of the inner ear. However, the molecular contributions of specific cell types remain poorly characterized. Therefore, we aimed to identify pH regulators in pendrin-expressing cells that may contribute to the homeostasis of endolymph pH and define the cellular pathogenic mechanisms that contribute to the dysregulation of cochlear endolymph pH in Slc26a4 We used single-cell RNA sequencing to identify both Slc26a4-expressing cells and Kcnj10-expressing cells in wild-type (WT, Slc26a4 We found that spindle cells, which express pendrin, contain extrinsic cellular components, a factor that enables cell-to-cell communication. In addition, the gene expression profile informed the pH of the spindle cells. Compared to WT, the transcriptional profiles in Slc26a4 Overall, cell isolation of stria vascularis from WT and Slc26a4
Sections du résumé
BACKGROUND
The primary pathological alterations of Pendred syndrome are endolymphatic pH acidification and luminal enlargement of the inner ear. However, the molecular contributions of specific cell types remain poorly characterized. Therefore, we aimed to identify pH regulators in pendrin-expressing cells that may contribute to the homeostasis of endolymph pH and define the cellular pathogenic mechanisms that contribute to the dysregulation of cochlear endolymph pH in Slc26a4
METHODS
We used single-cell RNA sequencing to identify both Slc26a4-expressing cells and Kcnj10-expressing cells in wild-type (WT, Slc26a4
RESULTS
We found that spindle cells, which express pendrin, contain extrinsic cellular components, a factor that enables cell-to-cell communication. In addition, the gene expression profile informed the pH of the spindle cells. Compared to WT, the transcriptional profiles in Slc26a4
CONCLUSION
Overall, cell isolation of stria vascularis from WT and Slc26a4
Identifiants
pubmed: 37322474
doi: 10.1186/s12920-023-01549-0
pii: 10.1186/s12920-023-01549-0
pmc: PMC10268361
doi:
Substances chimiques
Anion Transport Proteins
0
Sulfate Transporters
0
RNA
63231-63-0
Slc26a4 protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
133Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC002842
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC012049
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC017955
Pays : United States
Informations de copyright
© 2023. The Author(s).
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