Crystal structure of the monocupin ring-cleaving dioxygenase 5-nitrosalicylate 1,2-dioxygenase from Bradyrhizobium sp.
5-nitrosalicylate 1,2-dioxygenase
Bradyrhizobium sp JS329
X-ray crystallography
aromatic catabolism
cupins
dioxygenases
substrate specificity
Journal
Acta crystallographica. Section D, Structural biology
ISSN: 2059-7983
Titre abrégé: Acta Crystallogr D Struct Biol
Pays: United States
ID NLM: 101676043
Informations de publication
Date de publication:
01 Jul 2023
01 Jul 2023
Historique:
received:
21
03
2023
accepted:
14
05
2023
medline:
30
6
2023
pubmed:
16
6
2023
entrez:
16
6
2023
Statut:
ppublish
Résumé
5-Nitrosalicylate 1,2-dioxygenase (5NSDO) is an iron(II)-dependent dioxygenase involved in the aerobic degradation of 5-nitroanthranilic acid by the bacterium Bradyrhizobium sp. It catalyzes the opening of the 5-nitrosalicylate aromatic ring, a key step in the degradation pathway. Besides 5-nitrosalicylate, the enzyme is also active towards 5-chlorosalicylate. The X-ray crystallographic structure of the enzyme was solved at 2.1 Å resolution by molecular replacement using a model from the AI program AlphaFold. The enzyme crystallized in the monoclinic space group P2
Identifiants
pubmed: 37326584
pii: S2059798323004199
doi: 10.1107/S2059798323004199
pmc: PMC10306065
doi:
Substances chimiques
Dioxygenases
EC 1.13.11.-
Iron
E1UOL152H7
Ferrous Compounds
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
632-640Informations de copyright
open access.
Références
Mol Microbiol. 2006 Sep;61(6):1469-84
pubmed: 16930152
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Microbiol Mol Biol Rev. 2010 Jun;74(2):250-72
pubmed: 20508249
J Biol Chem. 1990 Dec 25;265(36):22187-96
pubmed: 2266121
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
J Comput Chem. 2010 Jan 30;31(2):455-61
pubmed: 19499576
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Protein Sci. 2008 Aug;17(8):1362-73
pubmed: 18505738
Acta Crystallogr D Struct Biol. 2022 Jan 1;78(Pt 1):1-13
pubmed: 34981757
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Biochemistry. 2005 May 31;44(21):7632-43
pubmed: 15909978
J Comput Chem. 2015 May 15;36(13):996-1007
pubmed: 25824339
Proteins. 2021 Dec;89(12):1687-1699
pubmed: 34218458
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
pubmed: 20057045
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2256-68
pubmed: 15572779
J Biol Chem. 2004 Sep 3;279(36):37250-60
pubmed: 15220336
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Biochemistry. 2005 Jan 11;44(1):193-201
pubmed: 15628860
Proteins. 2021 Dec;89(12):1752-1769
pubmed: 34387010
J Mol Biol. 2008 Jul 25;380(5):856-68
pubmed: 18572191
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Structure. 2011 Jun 8;19(6):844-58
pubmed: 21645855
Acta Crystallogr D Biol Crystallogr. 1993 Jan 1;49(Pt 1):129-47
pubmed: 15299554
J Bacteriol. 2011 Jun;193(12):3057-63
pubmed: 21498645
Angew Chem Int Ed Engl. 2016 Jan 18;55(3):1168-72
pubmed: 26596241
Curr Opin Struct Biol. 2008 Dec;18(6):644-9
pubmed: 19007887
Protein Sci. 2021 Apr;30(4):728-734
pubmed: 33625752
Appl Environ Microbiol. 2012 Apr;78(8):2505-14
pubmed: 22287012
Appl Environ Microbiol. 2010 Mar;76(5):1417-22
pubmed: 20081004
J Struct Biol. 2012 Feb;177(2):431-8
pubmed: 22155290
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):386-94
pubmed: 21460457
Nucleic Acids Res. 2022 Jan 7;50(D1):D439-D444
pubmed: 34791371
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Crit Rev Biochem Mol Biol. 2006 Jul-Aug;41(4):241-67
pubmed: 16849108
Biochim Biophys Acta. 2015 Oct;1854(10 Pt A):1425-37
pubmed: 26093111
J Bacteriol. 2001 Dec;183(23):6936-42
pubmed: 11698383
J Struct Biol. 2012 Dec;180(3):563-71
pubmed: 22960182
Phytochemistry. 2004 Jan;65(1):7-17
pubmed: 14697267
FEBS J. 2013 Apr;280(7):1643-52
pubmed: 23384287
Biochim Biophys Acta Proteins Proteom. 2017 May;1865(5):520-530
pubmed: 28232026