BCAT1 controls embryonic neural stem cells proliferation and differentiation in the upper layer neurons.
BCAT1
Cerebral cortex
Neural progenitor cells
Upper layer neurons
Journal
Molecular brain
ISSN: 1756-6606
Titre abrégé: Mol Brain
Pays: England
ID NLM: 101468876
Informations de publication
Date de publication:
21 06 2023
21 06 2023
Historique:
received:
26
12
2022
accepted:
10
06
2023
medline:
23
6
2023
pubmed:
22
6
2023
entrez:
21
6
2023
Statut:
epublish
Résumé
The regulation of neural stem cell (NSC) proliferation and differentiation during brain development is a precisely controlled process, with the production of different neuronal subtypes governed by strict timelines. Glutamate is predominantly used as a neurotransmitter by the subtypes of neurons in the various layers of the cerebral cortex. The expression pattern of BCAT1, a gene involved in glutamate metabolism, in the different layers of neurons has yet to be fully understood. Using single-cell data, we have identified seven different states of NSCs and found that state 4 is closely associated with the development of projection neurons. By inferring the developmental trajectory of different neuronal subtypes from NSC subsets of this state, we discovered that BCAT1 is involved in the regulation of NSC proliferation and differentiation and is specifically highly expressed in layer II/III and IV neurons. Suppression of BCAT1 through shRNA resulted in a reduction in NSC proliferation and an abnormal development of layer II/III and IV neurons. These findings provide new insights into the role of BCAT1 in the regulation of NSC behavior and neuronal development.
Identifiants
pubmed: 37344908
doi: 10.1186/s13041-023-01044-8
pii: 10.1186/s13041-023-01044-8
pmc: PMC10283284
doi:
Substances chimiques
Glutamic Acid
3KX376GY7L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
53Informations de copyright
© 2023. The Author(s).
Références
J Nutr. 2006 Jan;136(1 Suppl):207S-11S
pubmed: 16365084
Nature. 2000 Jan 20;403(6767):316-21
pubmed: 10659851
Oncogene. 2017 Jul 20;36(29):4124-4134
pubmed: 28319069
J Neurosci. 2003 Apr 1;23(7):2840-50
pubmed: 12684471
Cell Tissue Res. 2001 Aug;305(2):187-202
pubmed: 11545256
J Neurochem. 2002 Sep;82(6):1367-75
pubmed: 12354284
Oncotarget. 2015 Oct 13;6(31):31522-43
pubmed: 26372729
Liver Int. 2016 Dec;36(12):1836-1847
pubmed: 27246112
Science. 1992 Oct 23;258(5082):597-603
pubmed: 1329206
J Neurosci. 2007 Apr 4;27(14):3813-22
pubmed: 17409246
Nature. 2017 May 25;545(7655):500-504
pubmed: 28514443
Nat Commun. 2019 Apr 23;10(1):1903
pubmed: 31015418
ScientificWorldJournal. 2008 Jul 13;8:621-42
pubmed: 18661051
Prog Neurobiol. 1990;35(4):245-96
pubmed: 1980745
Neuron. 2004 Mar 25;41(6):881-90
pubmed: 15046721
Pharmacol Rev. 1999 Mar;51(1):7-61
pubmed: 10049997
J Nutr. 2006 Jan;136(1 Suppl):324S-30S
pubmed: 16365107
FEBS Lett. 2017 Dec;591(24):3978-3992
pubmed: 29194577
Curr Top Med Chem. 2006;6(10):949-60
pubmed: 16787269
Nat Med. 2013 Jul;19(7):901-908
pubmed: 23793099
Science. 2019 May 10;364(6440):
pubmed: 31073041
J Neurosci Res. 2007 Nov 15;85(15):3347-58
pubmed: 17847118
Front Endocrinol (Lausanne). 2013 May 27;4:59
pubmed: 23750153
Annu Rev Neurosci. 2009;32:149-84
pubmed: 19555289
Am J Physiol Endocrinol Metab. 2004 Jan;286(1):E64-76
pubmed: 12965870
Ultraschall Med. 2018 Apr;39(2):181-189
pubmed: 29621826
Int Rev Cell Mol Biol. 2018;336:223-320
pubmed: 29413892
Science. 2016 Sep 9;353(6304):1161-5
pubmed: 27609895
J Nutr. 2001 Mar;131(3):846S-850S
pubmed: 11238772