Single-cell A/B testing for cell-cell communication.

Cell Communication Coculture Techniques Microglia Feedback

Journal

Cell systems
ISSN: 2405-4720
Titre abrégé: Cell Syst
Pays: United States
ID NLM: 101656080

Informations de publication

Date de publication:
21 Jun 2023
Historique:
received: 19 05 2023
revised: 20 05 2023
accepted: 22 05 2023
medline: 26 6 2023
pubmed: 23 6 2023
entrez: 22 6 2023
Statut: ppublish

Résumé

A new method developed by Francisco Quintana's group, systematic perturbation of encapsulated associated cells followed by sequencing (SPEAC-seq), applies a CRISPR screen to co-cultured interacting cells to identify the ligands mediating cell-cell communication. Using this approach, the authors discover the molecular basis of a microglia-astrocyte feedback loop that suppresses neuroinflammatory disease.

Identifiants

DOI: 10.1016/j.cels.2023.05.006 PMID: 37348460
pubmed: 37348460
pii: S2405-4712(23)00150-3
doi: 10.1016/j.cels.2023.05.006
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

428-429

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Caitlin M Aamodt (CM)

Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA.

Nathan E Lewis (NE)

Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA; Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA. Electronic address: nlewisres@ucsd.edu.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Communication cellulaire › Single-cell A/B testing for cell-cell communication.
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de coculture › Single-cell A/B testing for cell-cell communication.
questionsmedicales.fr › Cellules › Névroglie › Microglie › Single-cell A/B testing for cell-cell communication.
questionsmedicales.fr › Sciences de l'information › Analyse des systèmes › Recherche opérationnelle › Rétroaction › Single-cell A/B testing for cell-cell communication.