Isolation and characterization of the gene HvFAR1 encoding acyl-CoA reductase from the cer-za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions.
Hordeum
/ genetics
Plant Leaves
/ metabolism
Water
/ metabolism
Saccharomyces cerevisiae
/ metabolism
Arabidopsis
/ genetics
Waxes
/ metabolism
Mutation
/ genetics
Plant Epidermis
/ metabolism
Nuclear Proteins
/ metabolism
Arabidopsis Proteins
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Cyclin-Dependent Kinase Inhibitor Proteins
/ genetics
barley
cuticle
cutin
eceriferum
permeability
wax
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
07
02
2023
accepted:
16
05
2023
medline:
3
8
2023
pubmed:
23
6
2023
entrez:
22
6
2023
Statut:
ppublish
Résumé
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer-za.227 and cer-ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown. Cuticular waxes and permeabilities were measured in cer-za.227 and cer-ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer-za alleles were generated by genome editing. The CER-ZA protein was characterized after expression in yeast and Arabidopsis cer4-3. Cer-za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl-CoA reductase (FAR1). The cer-ye.267 mutation is located to HORVU4Hr1G063420 encoding β-ketoacyl-CoA synthase (KAS1) and is allelic to cer-zh.54. The amounts of intracuticular waxes were strongly decreased in cer-ye.267. The cuticular water loss and permeability of cer-za.227 were similar to wild-type (WT), but were increased in cer-ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration. The differential decrease in intracuticular waxes between cer-za.227 and cer-ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.
Substances chimiques
hexadecanal dehydrogenase (acylating)
EC 1.2.1.42
Water
059QF0KO0R
Waxes
0
FAR1 protein, Arabidopsis
0
Nuclear Proteins
0
Arabidopsis Proteins
0
FAR1 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Cyclin-Dependent Kinase Inhibitor Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1903-1918Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M018466/1
Pays : United Kingdom
Informations de copyright
© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
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