Maternal acrylamide exposure changes intestinal epithelium, immunolocalization of leptin and ghrelin and their receptors, and gut barrier in weaned offspring.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 06 2023
Historique:
received: 01 02 2023
accepted: 23 06 2023
medline: 26 6 2023
pubmed: 25 6 2023
entrez: 24 6 2023
Statut: epublish

Résumé

Acrylamide (ACR) is an amide formed as a byproduct in many heat-processed starchy-rich foods. In utero ACR exposure has been associated with restricted fetal growth, but its effects of postnatal functional development of small intestine is completely unknown. The current study investigated the time- and segment-dependent effects of prenatal ACR exposure on morphological and functional development of small intestine in weaned rat offspring. Four groups of pregnant female Wistar rats were exposed to ACR (3 mg/kg b.w./day) for 0, 5, 10 and 15 days during pregnancy. Basal intestinal morphology, immunolocalization of gut hormones responsible for food intake and proteins of intestinal barrier, activity of the intestinal brush border disaccharidases, apoptosis and proliferation in intestinal mucosa were analyzed in offspring at weaning (postnatal day 21). The results showed that in utero ACR exposure disturbs offspring gut structural and functional postnatal development in a time- and segment-depended manner and even a short prenatal exposure to ACR resulted in changes in intestinal morphology, immunolocalization of leptin and ghrelin and their receptors, barrier function, activity of gut enzymes and upregulation of apoptosis and proliferation. In conclusion, prenatal ACR exposure disturbed the proper postnatal development of small intestine.

Identifiants

pubmed: 37355724
doi: 10.1038/s41598-023-37590-3
pii: 10.1038/s41598-023-37590-3
pmc: PMC10290718
doi:

Substances chimiques

Acrylamide 20R035KLCI
Ghrelin 0
Leptin 0
Receptors, Leptin 0
Receptors, Ghrelin 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

10286

Informations de copyright

© 2023. The Author(s).

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Auteurs

Siemowit Muszyński (S)

Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, Akademicka St. 13, 20-950, Lublin, Poland.

Monika Hułas-Stasiak (M)

Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033, Lublin, Poland.

Piotr Dobrowolski (P)

Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka St. 19, 20-033, Lublin, Poland.

Marcin B Arciszewski (MB)

Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland.

Ligia Hiżewska (L)

Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland.

Janine Donaldson (J)

School of Physiology, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, Johannesburg, 2193, South Africa.

Sylwia Mozel (S)

Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland.

Karol Rycerz (K)

Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland.

Małgorzata Kapica (M)

Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland.

Iwona Puzio (I)

Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland.

Ewa Tomaszewska (E)

Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka St. 12, 20-950, Lublin, Poland. ewaRST@interia.pl.

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