The HIV-1 capsid core is an opportunistic nuclear import receptor.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 06 2023
24 06 2023
Historique:
received:
16
02
2018
accepted:
01
06
2023
medline:
26
6
2023
pubmed:
25
6
2023
entrez:
24
6
2023
Statut:
epublish
Résumé
The movement of viruses and other large macromolecular cargo through nuclear pore complexes (NPCs) is poorly understood. The human immunodeficiency virus type 1 (HIV-1) provides an attractive model to interrogate this process. HIV-1 capsid (CA), the chief structural component of the viral core, is a critical determinant in nuclear transport of the virus. HIV-1 interactions with NPCs are dependent on CA, which makes direct contact with nucleoporins (Nups). Here we identify Nup35, Nup153, and POM121 to coordinately support HIV-1 nuclear entry. For Nup35 and POM121, this dependence was dependent cyclophilin A (CypA) interaction with CA. Mutation of CA or removal of soluble host factors changed the interaction with the NPC. Nup35 and POM121 make direct interactions with HIV-1 CA via regions containing phenylalanine glycine motifs (FG-motifs). Collectively, these findings provide additional evidence that the HIV-1 CA core functions as a macromolecular nuclear transport receptor (NTR) that exploits soluble host factors to modulate NPC requirements during nuclear invasion.
Identifiants
pubmed: 37355754
doi: 10.1038/s41467-023-39146-5
pii: 10.1038/s41467-023-39146-5
pmc: PMC10290713
doi:
Substances chimiques
Nuclear Pore Complex Proteins
0
Capsid Proteins
0
POM121 protein, human
0
Membrane Glycoproteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3782Subventions
Organisme : NIAID NIH HHS
ID : R01 AI087390
Pays : United States
Organisme : NIGMS NIH HHS
ID : P50 GM103368
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI111809
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI120860
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI117839
Pays : United States
Organisme : NIDA NIH HHS
ID : DP1 DA034990
Pays : United States
Organisme : NIAID NIH HHS
ID : U54 AI170856
Pays : United States
Informations de copyright
© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.
Références
Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):
pubmed: 33649225
Antimicrob Agents Chemother. 2013 Oct;57(10):4622-31
pubmed: 23817385
Science. 2008 Feb 15;319(5865):921-6
pubmed: 18187620
J Virol. 2007 Nov;81(21):11946-56
pubmed: 17715216
J Virol. 2012 Apr;86(8):4708-14
pubmed: 22301145
Cell Rep. 2020 Sep 29;32(13):108201
pubmed: 32997983
Retrovirology. 2014 Jan 30;11:11
pubmed: 24479545
J Cell Biol. 2011 Jul 11;194(1):27-37
pubmed: 21727197
Retrovirology. 2013 Feb 15;10:20
pubmed: 23414560
Viruses. 2013 Oct 07;5(10):2483-511
pubmed: 24103892
J Virol. 2004 Dec;78(23):12800-8
pubmed: 15542632
EMBO J. 2001 Jun 15;20(12):3272-81
pubmed: 11406603
Elife. 2018 Aug 07;7:
pubmed: 30084827
Mol Cell Biol. 2000 Oct;20(20):7798-812
pubmed: 11003674
J Virol. 1996 Jun;70(6):3551-60
pubmed: 8648689
Mol Syst Biol. 2013;9:648
pubmed: 23511206
J Virol. 1998 Jul;72(7):6004-13
pubmed: 9621063
Cell Rep. 2020 Mar 17;30(11):3766-3777.e6
pubmed: 32187548
PLoS Pathog. 2013;9(10):e1003693
pubmed: 24130490
J Virol. 2002 Dec;76(23):11889-903
pubmed: 12414931
PLoS Pathog. 2012;8(8):e1002896
pubmed: 22956906
Nat Commun. 2020 Nov 26;11(1):6165
pubmed: 33244008
Cell Host Microbe. 2010 Mar 18;7(3):221-33
pubmed: 20227665
Nat Commun. 2022 Oct 6;13(1):5879
pubmed: 36202818
PLoS Pathog. 2014 Oct 30;10(10):e1004459
pubmed: 25356722
J Virol. 2005 Jan;79(1):176-83
pubmed: 15596813
Cell. 2021 Feb 18;184(4):1032-1046.e18
pubmed: 33571428
Nat Microbiol. 2021 Apr;6(4):435-444
pubmed: 33649557
Nat Microbiol. 2019 Dec;4(12):2044-2051
pubmed: 31636416
J Virol. 2001 Jul;75(14):6537-46
pubmed: 11413321
Nat Commun. 2020 Jul 14;11(1):3505
pubmed: 32665593
Virology. 2001 Mar 30;282(1):154-67
pubmed: 11259198
PLoS Pathog. 2017 Aug 21;13(8):e1006570
pubmed: 28827840
J Biol Chem. 1996 Sep 20;271(38):23478-86
pubmed: 8798556
J Virol. 2018 Sep 12;92(19):
pubmed: 29997211
PLoS Pathog. 2010 Dec 09;6(12):e1001220
pubmed: 21170360
J Virol. 2012 Apr;86(7):3851-60
pubmed: 22301135
J Exp Med. 1999 Jun 7;189(11):1735-46
pubmed: 10359577
EMBO J. 2012 Oct 17;31(20):4072-84
pubmed: 22960634
Nat Med. 2019 Sep;25(9):1377-1384
pubmed: 31501601
Nat Microbiol. 2020 Sep;5(9):1088-1095
pubmed: 32483230
J Virol. 2011 Aug;85(15):7818-27
pubmed: 21593146
Virology. 2013 May 25;440(1):8-18
pubmed: 23523133
PLoS Pathog. 2014 Feb 20;10(2):e1003969
pubmed: 24586169
Cell. 2012 May 25;149(5):1035-47
pubmed: 22632968
PLoS Pathog. 2011 Dec;7(12):e1002439
pubmed: 22174692
Cell. 1993 Jun 18;73(6):1067-78
pubmed: 8513493
PLoS Pathog. 2007 Oct 26;3(10):1502-10
pubmed: 17967060
Science. 2007 Nov 30;318(5855):1412-6
pubmed: 18048681
Cell. 2008 Oct 3;135(1):49-60
pubmed: 18854154
iScience. 2021 Dec 09;25(1):103593
pubmed: 35005542
Elife. 2019 Jan 23;8:
pubmed: 30672737
J Exp Med. 1991 Dec 1;174(6):1477-82
pubmed: 1720811
Elife. 2021 Apr 27;10:
pubmed: 33904396