Substrate-dependent metabolomic signatures of myeloperoxidase activity in airway epithelial cells: Implications for early cystic fibrosis lung disease.

Humans Child, Preschool Thiocyanates / metabolism Peroxidase / metabolism Cystic Fibrosis Bromides Chlorides Oxidants / metabolism Antioxidants Hypochlorous Acid / metabolism Epithelial Cells / metabolism Metabolomics
Cystic fibrosis Dehydromethionine Hypothiocyanite Metabolomics Myeloperoxidase Oxidative stress

Journal

Free radical biology & medicine
ISSN: 1873-4596
Titre abrégé: Free Radic Biol Med
Pays: United States
ID NLM: 8709159

Informations de publication

Date de publication:
09 2023
Historique:
received: 13 05 2023
revised: 14 06 2023
accepted: 23 06 2023
pmc-release: 01 09 2024
medline: 18 8 2023
pubmed: 26 6 2023
entrez: 25 6 2023
Statut: ppublish

Résumé

Myeloperoxidase (MPO) is released by neutrophils in inflamed tissues. MPO oxidizes chloride, bromide, and thiocyanate to produce hypochlorous acid (HOCl), hypobromous acid (HOBr), and hypothiocyanous acid (HOSCN), respectively. These oxidants are toxic to pathogens, but may also react with host cells to elicit biological activity and potential toxicity. In cystic fibrosis (CF) and related diseases, increased neutrophil inflammation leads to increased airway MPO and airway epithelial cell (AEC) exposure to its oxidants. In this study, we investigated how equal dose-rate exposures of MPO-derived oxidants differentially impact the metabolome of human AECs (BEAS-2B cells). We utilized enzymatic oxidant production with rate-limiting glucose oxidase (GOX) coupled to MPO, and chloride, bromide (Br

Identifiants

DOI: 10.1016/j.freeradbiomed.2023.06.021 PMID: 37356776 PMC: PMC10513041
pubmed: 37356776
pii: S0891-5849(23)00508-7
doi: 10.1016/j.freeradbiomed.2023.06.021
pmc: PMC10513041
mid: NIHMS1919984
pii:
doi:

Substances chimiques

thiocyanate O748SU14OM
Thiocyanates 0
hypobromous acid GHT9BV419J
Peroxidase EC 1.11.1.7
Bromides 0
Chlorides 0
Oxidants 0
Antioxidants 0
Hypochlorous Acid 712K4CDC10

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

180-190

Subventions

Organisme : NIEHS NIH HHS
ID : R01 ES031980
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL150658
Pays : United States
Organisme : NIEHS NIH HHS
ID : R21 ES031824
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM135060
Pays : United States

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Susan O Kim (SO)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA.

Joseph P Shapiro (JP)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA.

Kirsten A Cottrill (KA)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA.

Genoah L Collins (GL)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA.

Shivanthan Shanthikumar (S)

Respiratory and Sleep Medicine, Royal Children's Hospital, Parkville, VIC, Australia; Respiratory Diseases, Murdoch Children's Research Institute, Parkville, VIC, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.

Padma Rao (P)

Medical Imaging, Royal Children's Hospital, Parkville, VIC, Australia.

Sarath Ranganathan (S)

Respiratory and Sleep Medicine, Royal Children's Hospital, Parkville, VIC, Australia; Respiratory Diseases, Murdoch Children's Research Institute, Parkville, VIC, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.

Stephen M Stick (SM)

Telethon Kids Institute, Perth, Western Australia, Australia.

Michael L Orr (ML)

Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Emory University, Atlanta, GA, USA.

Anne M Fitzpatrick (AM)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA; Children's Healthcare of Atlanta, Atlanta, GA, USA.

Young-Mi Go (YM)

Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Emory University, Atlanta, GA, USA.

Dean P Jones (DP)

Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Emory University, Atlanta, GA, USA.

Rabindra M Tirouvanziam (RM)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA; Children's Healthcare of Atlanta, Atlanta, GA, USA.

Joshua D Chandler (JD)

Department of Pediatrics, Division of Pulmonary, Asthma, Cystic Fibrosis, and Sleep, Emory University, Atlanta, GA, USA; Department of Medicine, Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Emory University, Atlanta, GA, USA; Children's Healthcare of Atlanta, Atlanta, GA, USA. Electronic address: joshua.chandler@emory.edu.

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questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Personnes › Tranches d'âge › Enfant › Enfant d'âge préscolaire › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Composés chimiques organiques › Composés du soufre › Thiocyanates › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Peroxidases › Myeloperoxidase › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Malformations et maladies congénitales, héréditaires et néonatales › Maladies néonatales › Mucoviscidose › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Produits chimiques inorganiques › Électrolytes › Ions › Anions › Bromures › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Produits chimiques inorganiques › Électrolytes › Ions › Anions › Chlorures › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Actions chimiques et utilisations › Actions toxiques › Noxas › Oxydants › Substrate-dependent metabolomic signatures of...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Antioxydants › Substrate-dependent metabolomic signatures of...
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questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Substrate-dependent metabolomic signatures of...