Phase separation-based visualization of protein-protein interactions and kinase activities in plants.
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
01 09 2023
01 09 2023
Historique:
received:
06
01
2023
accepted:
23
06
2023
pmc-release:
28
06
2024
medline:
5
9
2023
pubmed:
28
6
2023
entrez:
28
6
2023
Statut:
ppublish
Résumé
Protein activities depend heavily on protein complex formation and dynamic posttranslational modifications, such as phosphorylation. The dynamic nature of protein complex formation and posttranslational modifications is notoriously difficult to monitor in planta at cellular resolution, often requiring extensive optimization. Here, we generated and exploited the SYnthetic Multivalency in PLants (SYMPL)-vector set to assay protein-protein interactions (PPIs) (separation of phases-based protein interaction reporter) and kinase activities (separation of phases-based activity reporter of kinase) in planta, based on phase separation. This technology enabled easy detection of inducible, binary and ternary PPIs among cytoplasmic and nuclear proteins in plant cells via a robust image-based readout. Moreover, we applied the SYMPL toolbox to develop an in vivo reporter for SNF1-related kinase 1 activity, allowing us to visualize tissue-specific, dynamic SnRK1 activity in stable transgenic Arabidopsis (Arabidopsis thaliana) plants. The SYMPL cloning toolbox provides a means to explore PPIs, phosphorylation, and other posttranslational modifications with unprecedented ease and sensitivity.
Identifiants
pubmed: 37378595
pii: 7209236
doi: 10.1093/plcell/koad188
pmc: PMC10473206
doi:
Substances chimiques
Arabidopsis Proteins
0
SnRK1 protein, Arabidopsis
EC 2.7.11.1
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3280-3302Subventions
Organisme : European Research Council
ID : 682436
Pays : International
Informations de copyright
© American Society of Plant Biologists 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Déclaration de conflit d'intérêts
Conflict of interest statement. None declared.
Références
Plant Cell. 2019 Jul;31(7):1614-1632
pubmed: 31123051
Front Mol Biosci. 2021 May 07;8:673038
pubmed: 34026847
Nat Protoc. 2006;1(2):968-71
pubmed: 17406331
Elife. 2017 Jun 19;6:
pubmed: 28628006
Nat Plants. 2022 Jul;8(7):778-791
pubmed: 35817823
Proc Natl Acad Sci U S A. 2001 May 8;98(10):5596-601
pubmed: 11344301
Nature. 2020 May;581(7807):209-214
pubmed: 32405004
J Biol Chem. 2005 Aug 26;280(34):30448-59
pubmed: 15772084
Nature. 2012 Mar 07;483(7389):336-40
pubmed: 22398450
Methods Mol Biol. 2011;754:195-218
pubmed: 21720954
Mol Cell. 2000 Nov;6(5):1169-82
pubmed: 11106755
Front Plant Sci. 2022 Apr 22;13:883847
pubmed: 35528934
Elife. 2020 May 28;9:
pubmed: 32463362
Nat Commun. 2015 Jul 09;6:7670
pubmed: 26158227
Plant Physiol. 2021 Dec 4;187(4):1985-2004
pubmed: 33905517
Science. 2011 May 27;332(6033):1071-6
pubmed: 21617073
Trends Cell Biol. 2020 Jan;30(1):4-14
pubmed: 31753533
BMC Biotechnol. 2009 Nov 20;9:96
pubmed: 19930574
J Biol Chem. 2002 Sep 6;277(36):32624-31
pubmed: 12077118
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Anal Chem. 2018 Dec 18;90(24):14287-14293
pubmed: 30431263
Structure. 2006 Mar;14(3):477-85
pubmed: 16531232
J Exp Bot. 2016 Apr;67(9):2745-60
pubmed: 27029354
Plants (Basel). 2022 Oct 06;11(19):
pubmed: 36235497
Mol Cell Proteomics. 2007 Jul;6(7):1226-38
pubmed: 17426018
CSH Protoc. 2008 Apr 01;2008:pdb.prot4995
pubmed: 21356813
Trends Plant Sci. 1999 Jul;4(7):287-291
pubmed: 10407445
Mol Biol Cell. 2011 Dec;22(23):4647-56
pubmed: 21976697
Ann Bot. 2016 Jan;117(1):9-24
pubmed: 26558398
Plant Cell. 2022 Jan 20;34(1):616-632
pubmed: 34755865
Dev Cell. 2020 Oct 12;55(1):69-83
pubmed: 33049212
Methods Mol Biol. 2009;479:189-202
pubmed: 19083187
Sci Rep. 2017 Apr 13;7:46380
pubmed: 28406179
Plant Cell. 2015 Apr;27(4):1185-99
pubmed: 25829440
Plant Direct. 2019 Jan 17;3(1):e00112
pubmed: 31245754
PLoS One. 2013 Dec 20;8(12):e83043
pubmed: 24376629
Mol Cell. 2018 Jan 18;69(2):347
pubmed: 29351851
Protein Pept Lett. 2018;25(8):728-733
pubmed: 30129399
Sci Adv. 2021 Feb 26;7(9):
pubmed: 33637534
Nat Plants. 2019 Mar;5(3):316-327
pubmed: 30833711
Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):15332-15342
pubmed: 32541020
Science. 2014 Oct 24;346(6208):485-8
pubmed: 25342807
Chem Rev. 2018 Dec 26;118(24):11707-11794
pubmed: 30550275
Mol Biomed. 2022 May 11;3(1):13
pubmed: 35543798
Plant J. 2019 Mar;97(5):970-983
pubmed: 30444549
Nat Plants. 2020 Nov;6(11):1345-1353
pubmed: 33077877
Semin Cell Dev Biol. 2011 Sep;22(7):663-72
pubmed: 21920446
Structure. 2000 Aug 15;8(8):841-50
pubmed: 10997903
Plant Physiol. 2010 Feb;152(2):553-65
pubmed: 20018602
New Phytol. 2015 Oct;208(1):269-79
pubmed: 25919910
Cell. 2021 Aug 5;184(16):4284-4298.e27
pubmed: 34233164
J Exp Bot. 2022 Nov 15;73(20):7055-7067
pubmed: 35861169
Curr Opin Cell Biol. 2001 Apr;13(2):131-8
pubmed: 11248545
Plant Cell. 2010 Apr;22(4):1264-80
pubmed: 20407024
Mol Cell. 2019 Oct 3;76(1):177-190.e5
pubmed: 31421981
Cell. 2016 Jul 28;166(3):651-663
pubmed: 27374333
Nat Protoc. 2014 Sep;9(9):2075-89
pubmed: 25101822
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Plant Cell. 2013 Oct;25(10):3871-84
pubmed: 24179127
FEBS Lett. 2002 Feb 20;513(1):124-8
pubmed: 11911891
Nat Protoc. 2008;3(11):1693-702
pubmed: 18846096
Nature. 2019 May;569(7755):265-269
pubmed: 31043738
Front Biosci (Landmark Ed). 2012 Jun 01;17(6):2140-57
pubmed: 22652769
Curr Opin Plant Biol. 2010 Oct;13(5):527-32
pubmed: 20638895
Science. 2007 Apr 20;316(5823):421-5
pubmed: 17446396
Plant Cell. 2023 Sep 1;35(9):3173-3186
pubmed: 36879427
Plant J. 2016 Sep;87(6):559-67
pubmed: 27227462
Plant Physiol. 2003 Jun;132(2):666-80
pubmed: 12805596
Cell Metab. 2011 Apr 6;13(4):476-486
pubmed: 21459332
Plant Cell. 2019 May;31(5):1171-1184
pubmed: 30872321
Sci Adv. 2023 Mar;9(9):eade3760
pubmed: 36857446
Mol Cell. 2011 Dec 23;44(6):878-92
pubmed: 22137581
J Exp Bot. 2019 Apr 15;70(8):2261-2274
pubmed: 30793201
Science. 2014 Oct 24;346(6208):481-485
pubmed: 25342806
Methods Mol Biol. 2014;1188:245-61
pubmed: 25059616
J Exp Bot. 2018 May 15;:
pubmed: 29767757
Biochemistry. 2002 Jan 29;41(4):1202-10
pubmed: 11802719
Biochem J. 2010 Mar 15;427(1):69-78
pubmed: 20141511
Trends Cell Biol. 2018 Jun;28(6):420-435
pubmed: 29602697
J Biol Chem. 2020 Aug 14;295(33):11420-11434
pubmed: 32461254
J Biol Chem. 2009 Oct 2;284(40):27425-37
pubmed: 19651772
Nat Protoc. 2015 Jan;10(1):169-87
pubmed: 25521792
Methods Protoc. 2018 Mar;1(1):
pubmed: 29451563
Chem Rev. 2007 Nov;107(11):5065-81
pubmed: 17949044
Traffic. 2003 Jul;4(7):491-501
pubmed: 12795694
J Am Chem Soc. 2005 Apr 6;127(13):4715-21
pubmed: 15796538
J Biol Chem. 2006 Jul 7;281(27):18816-24
pubmed: 16675442
J Exp Bot. 2016 Dec;67(22):6215-6252
pubmed: 27856705
J Exp Bot. 2021 May 4;72(10):3881-3901
pubmed: 33758916
Nat Methods. 2004 Dec;1(3):209-17
pubmed: 15782196
Plant Cell. 2023 Sep 1;35(9):3363-3382
pubmed: 37040611
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Nat Cell Biol. 2022 Jul;24(7):1029-1037
pubmed: 35711061
Front Plant Sci. 2018 May 15;9:640
pubmed: 29868093
Nat Commun. 2022 Jul 5;13(1):3856
pubmed: 35790710
Science. 1999 Feb 26;283(5406):1325-8
pubmed: 10037602
Curr Opin Plant Biol. 2019 Oct;51:29-36
pubmed: 31030062
Plant Cell. 2016 May;28(5):1002-8
pubmed: 27099259
Emerg Top Life Sci. 2020 Dec 11;4(3):307-329
pubmed: 33078839
Nat Chem Biol. 2021 May;17(5):549-557
pubmed: 33633378
Proc Natl Acad Sci U S A. 2021 Sep 14;118(37):
pubmed: 34504003
FEBS Lett. 2017 Nov;591(21):3625-3636
pubmed: 28940407
Protoplasma. 2014 Mar;251(2):383-94
pubmed: 24390247
Oncogene. 2007 Aug 13;26(37):5521-7
pubmed: 17694091
Plant J. 2006 Jul;47(2):211-23
pubmed: 16771775
Int J Mol Sci. 2019 Nov 05;20(21):
pubmed: 31694155
Cell. 2014 Feb 13;156(4):691-704
pubmed: 24529374
Plant J. 2012 Apr;70(1):118-28
pubmed: 22449047
Plant Cell. 2020 Aug;32(8):2582-2601
pubmed: 32471862
Elife. 2016 Jun 11;5:
pubmed: 27288545
Trends Biochem Sci. 2023 May;48(5):417-419
pubmed: 36621339
Biotechniques. 2012 Oct 02;53(5):311-14
pubmed: 23066669
Nat Plants. 2022 Nov;8(11):1245-1261
pubmed: 36376753
Front Plant Sci. 2019 May 10;10:503
pubmed: 31134102
Annu Rev Biophys. 2019 May 6;48:19-44
pubmed: 30786230
Plant Cell. 2002;14 Suppl:S339-54
pubmed: 12045287
Plant Methods. 2018 Aug 25;14:71
pubmed: 30159002
Plant Cell. 2021 May 31;33(4):1101-1117
pubmed: 33793859