Stochastic motion and transcriptional dynamics of pairs of distal DNA loci on a compacted chromosome.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
30 06 2023
30 06 2023
Historique:
pmc-release:
30
06
2024
medline:
3
7
2023
pubmed:
29
6
2023
entrez:
29
6
2023
Statut:
ppublish
Résumé
Chromosomes in the eukaryotic nucleus are highly compacted. However, for many functional processes, including transcription initiation, the pairwise motion of distal chromosomal elements such as enhancers and promoters is essential and necessitates dynamic fluidity. Here, we used a live-imaging assay to simultaneously measure the positions of pairs of enhancers and promoters and their transcriptional output while systematically varying the genomic separation between these two DNA loci. Our analysis reveals the coexistence of a compact globular organization and fast subdiffusive dynamics. These combined features cause an anomalous scaling of polymer relaxation times with genomic separation leading to long-ranged correlations. Thus, encounter times of DNA loci are much less dependent on genomic distance than predicted by existing polymer models, with potential consequences for eukaryotic gene expression.
Identifiants
pubmed: 37384691
doi: 10.1126/science.adf5568
pmc: PMC10439308
mid: NIHMS1922794
doi:
Substances chimiques
DNA
9007-49-2
Polymers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1357-1362Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM097275
Pays : United States
Organisme : NIDA NIH HHS
ID : U01 DA047730
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK127429
Pays : United States
Commentaires et corrections
Type : UpdateOf
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