Efficient and versatile multiplex prime editing in hexaploid wheat.
Hexaploid wheat
Multiplex genome editing
Prime editing
Reverse transcriptase
Journal
Genome biology
ISSN: 1474-760X
Titre abrégé: Genome Biol
Pays: England
ID NLM: 100960660
Informations de publication
Date de publication:
29 Jun 2023
29 Jun 2023
Historique:
received:
08
01
2023
accepted:
15
06
2023
medline:
3
7
2023
pubmed:
30
6
2023
entrez:
29
6
2023
Statut:
epublish
Résumé
Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A substitution into reverse transcriptase in the ePPEmax* architecture. ePPEplus enhances the efficiency by an average 33.0-fold and 6.4-fold compared to the original PPE and ePPE, respectively. Importantly, a robust multiplex prime editing platform is established for simultaneous editing of four to ten genes in protoplasts and up to eight genes in regenerated wheat plants at frequencies up to 74.5%, thus expanding the applicability of prime editors for stacking of multiple agronomic traits.
Identifiants
pubmed: 37386475
doi: 10.1186/s13059-023-02990-1
pii: 10.1186/s13059-023-02990-1
pmc: PMC10308706
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
156Subventions
Organisme : National Key Research and Development Program of China
ID : 2021YFF1000800
Organisme : National Natural Science Foundation of China
ID : 32270429
Organisme : Chinese Universities Scientific Fund
ID : 2023TC019
Organisme : Frontiers Science Center for Molecular Design Breeding
ID : 2022TC152
Organisme : Hainan Yazhou Bay Seed Laboratory
ID : B21HJ0504
Informations de copyright
© 2023. The Author(s).
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