The debranching enzyme Dbr1 regulates lariat turnover and intron splicing.

AQR Dbr1 RNA processing RNA splicing intron lariats lariat-seq spliceosome

Journal

Research square
Titre abrégé: Res Sq
Pays: United States
ID NLM: 101768035

Informations de publication

Date de publication:
13 Jun 2023
Historique:
pubmed: 3 7 2023
medline: 3 7 2023
entrez: 3 7 2023
Statut: epublish

Résumé

The majority of genic transcription is intronic. Introns are removed by splicing as branched lariat RNAs which require rapid recycling. The branch site is recognized during splicing catalysis and later debranched by Dbr1 in the rate-limiting step of lariat turnover. Through generation of the first viable

Identifiants

pubmed: 37398028
doi: 10.21203/rs.3.rs-2931976/v1
pmc: PMC10312976
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM105681
Pays : United States
Organisme : NCI NIH HHS
ID : F31 CA254143
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA193318
Pays : United States
Organisme : NCI NIH HHS
ID : P01 CA092584
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA091842
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM127472
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA227001
Pays : United States

Déclaration de conflit d'intérêts

DECLARATION OF INTERESTS The authors declare no competing interests. We disclose W.G.F. as the founder of Walah Scientific and member of the scientific advisory board for Remix Therapeutics.

Auteurs

Luke Buerer (L)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Nathaniel E Clark (NE)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Anastasia Welch (A)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Chaorui Duan (C)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Allison J Taggart (AJ)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Brittany A Townley (BA)

Department of Pathology & Immunology, Center for Genome Integrity, Washington University School of Medicine, St. Louis, MO 63110, USA.

Jing Wang (J)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Rachel Soemedi (R)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Stephen Rong (S)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Chien-Ling Lin (CL)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Yi Zeng (Y)

Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA.

Adam Katolik (A)

Department of Chemistry, McGill University, Montreal, QC H3A 0B8, Canada.

Jonathan P Staley (JP)

Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA.

Masad J Damha (MJ)

Department of Chemistry, McGill University, Montreal, QC H3A 0B8, Canada.

Nima Mosammaparast (N)

Department of Pathology & Immunology, Center for Genome Integrity, Washington University School of Medicine, St. Louis, MO 63110, USA.

William G Fairbrother (WG)

Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02903, USA.

Classifications MeSH