STIM1 signals through NFAT1 independently of Orai1 and SOCE to regulate breast cancer cell migration.
Breast cancer
Cell migration
Invasion
Metastatic
NFAT
Orai1
STIM1
Store-operated calcium entry
Journal
Cell calcium
ISSN: 1532-1991
Titre abrégé: Cell Calcium
Pays: Netherlands
ID NLM: 8006226
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
27
03
2023
revised:
25
06
2023
accepted:
27
06
2023
pubmed:
4
7
2023
medline:
4
7
2023
entrez:
3
7
2023
Statut:
ppublish
Résumé
Store-operated calcium entry (SOCE) contributes to several physiological and pathological conditions including transcription, secretion, immunodeficiencies, and cancer. SOCE has been shown to be important for breast cancer cell migration where knockdown of SOCE components (STIM1 or Orai1) decreases cancer metastasis. Here we show unexpectedly that complete knockout of STIM1 (STIM1-KO) using gene editing in metastatic MDA-MB-231 breast cancer cells results in faster migration and enhanced invasion capacity. In contrast, Orai1-KO cells, which have similar levels of SOCE inhibition as STIM1-KO, migrate slower than the parental cell line. This shows that the enhanced migration phenotype of STIM1-KO cells is not due to the loss of Ca
Identifiants
pubmed: 37399784
pii: S0143-4160(23)00091-X
doi: 10.1016/j.ceca.2023.102779
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102779Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no competing interests.