Quantitative Analysis of The High-Yield Hydrolysis of Kelp by Laminarinase and Alginate Lyase.
2D HSQC NMR
Glycoside Hydrolases
Nanostructure-initiator mass spectrometry (NIMS)
Polysaccharide lyases
bioenergy
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
17 10 2023
17 10 2023
Historique:
revised:
03
07
2023
received:
11
05
2023
medline:
23
10
2023
pubmed:
5
7
2023
entrez:
4
7
2023
Statut:
ppublish
Résumé
Kelp is an abundant, farmable biomass-containing laminarin and alginate as major polysaccharides, providing an excellent model substrate to study their deconstruction by simple enzyme mixtures. Our previous study showed strong reactivity of the glycoside hydrolase family 55 during hydrolysis of purified laminarin, raising the question of its reactivity with intact kelp. In this study, we determined that a combination of a single glycoside hydrolase family 55 β-1,3-exoglucanase with a broad-specificity alginate lyase from the polysaccharide lyase family 18 gives efficient hydrolysis of untreated kelp to a mixture of simple sugars, that is, glucose, gentiobiose, mannitol-end glucose, and mannuronic and guluronic acids and their soluble oligomers. Quantitative assignments from nanostructure initiator mass spectrometry (NIMS) and 2D HSQC NMR spectroscopy and analysis of the reaction time-course are provided. The data suggest that binary combinations of enzymes targeted to the unique polysaccharide composition of marine biomass are sufficient to deconstruct kelp into soluble sugars for microbial fermentation.
Identifiants
pubmed: 37402642
doi: 10.1002/cbic.202300357
doi:
Substances chimiques
poly(beta-D-mannuronate) lyase
EC 4.2.2.3
Cellulases
EC 3.2.1.-
Polysaccharide-Lyases
EC 4.2.2.-
Polysaccharides
0
Glucose
IY9XDZ35W2
Glycoside Hydrolases
EC 3.2.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300357Informations de copyright
© 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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