Whole Genome Sequencing in Advanced Lung Cancer can be Performed Using Diff-Quik Cytology Smears Derived from Endobronchial Ultrasound, Transbronchial Needle Aspiration (EBUS TBNA).
Cytology
Endobronchial ultrasound–guided transbronchial needle aspiration (EBUS TBNA)
Lung cancer
Molecular diagnostics
Whole genome sequencing
Journal
Lung
ISSN: 1432-1750
Titre abrégé: Lung
Pays: United States
ID NLM: 7701875
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
received:
22
04
2023
accepted:
25
06
2023
medline:
24
8
2023
pubmed:
5
7
2023
entrez:
5
7
2023
Statut:
ppublish
Résumé
Maximising alternative sample types for genomics in advanced lung cancer is important because bronchoscopic samples may sometimes be insufficient for this purpose. Further, the clinical applications of comprehensive molecular analysis such as whole genome sequencing (WGS) are rapidly developing. Diff-Quik cytology smears from EBUS TBNA is an alternative source of DNA, but its feasibility for WGS has not been previously demonstrated. Diff-Quik smears were collected along with research cell pellets. Tumour content of smears were compared to research cell pellets from 42 patients, which showed good correlation (Spearman correlation 0.85, P < 0.0001). A subset of eight smears underwent WGS, which presented similar mutation profiles to WGS of the matched cell pellet. DNA yield was predicted using a regression equation of the smears cytology features, which correctly predicted DNA yield > 1500 ng in 7 out of 8 smears. WGS of commonly collected Diff-Quik slides is feasible and their DNA yield can be predicted.
Identifiants
pubmed: 37405466
doi: 10.1007/s00408-023-00631-9
pii: 10.1007/s00408-023-00631-9
pmc: PMC10444633
doi:
Substances chimiques
Diff Quik
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
407-413Informations de copyright
© 2023. Crown.
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