Spermine and spermidine bind CXCR4 and inhibit CXCR4- but not CCR5-tropic HIV-1 infection.


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
07 07 2023
Historique:
medline: 7 7 2023
pubmed: 5 7 2023
entrez: 5 7 2023
Statut: ppublish

Résumé

Semen is an important vector for sexual HIV-1 transmission. Although CXCR4-tropic (X4) HIV-1 may be present in semen, almost exclusively CCR5-tropic (R5) HIV-1 causes systemic infection after sexual intercourse. To identify factors that may limit sexual X4-HIV-1 transmission, we generated a seminal fluid-derived compound library and screened it for antiviral agents. We identified four adjacent fractions that blocked X4-HIV-1 but not R5-HIV-1 and found that they all contained spermine and spermidine, abundant polyamines in semen. We showed that spermine, which is present in semen at concentrations up to 14 mM, binds CXCR4 and selectively inhibits cell-free and cell-associated X4-HIV-1 infection of cell lines and primary target cells at micromolar concentrations. Our findings suggest that seminal spermine restricts sexual X4-HIV-1 transmission.

Identifiants

pubmed: 37406129
doi: 10.1126/sciadv.adf8251
pmc: PMC10321752
doi:

Substances chimiques

Spermidine U87FK77H25
Spermine 2FZ7Y3VOQX
CXCR4 protein, human 0
Receptors, CXCR4 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadf8251

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Auteurs

Mirja Harms (M)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

Nikaïa Smith (N)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.
CNRS UMR-8601, Université Paris Cité, 75006 Paris, France.
Institut Cochin, Inserm U1016, CNRS UMR8104, Université Paris-Cité, 75014 Paris, France.

Mingyu Han (M)

Institut Cochin, Inserm U1016, CNRS UMR8104, Université Paris-Cité, 75014 Paris, France.

Rüdiger Groß (R)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

Pascal von Maltitz (P)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

Christina Stürzel (C)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

Yasser B Ruiz-Blanco (YB)

Computational Biochemistry, Center of Medical Biotechnology, University of Duisburg-Essen, Universitätsstr. 2, 45141 Essen, Germany.

Yasser Almeida-Hernández (Y)

Computational Bioengineering, Department of Biochemical and Chemical Engineering, Emil-Figge Str. 66., 44227 Dortmund, Germany.

Armando Rodriguez-Alfonso (A)

Core Facility Functional Peptidomics, Ulm University Medical Center, 89081 Ulm, Germany.
Core Unit Mass Spectrometry and Proteomics, Ulm University, 89081 Ulm, Germany.

Dominique Cathelin (D)

CNRS UMR-8601, Université Paris Cité, 75006 Paris, France.
Chemistry and Biology, Modeling and Immunology for Therapy (CBMIT), Paris, France.

Birgit Caspar (B)

CNRS UMR-8601, Université Paris Cité, 75006 Paris, France.
Chemistry and Biology, Modeling and Immunology for Therapy (CBMIT), Paris, France.

Bouceba Tahar (B)

Sorbonne University, CNRS, Institut de Biologie Paris-Seine (IBPS), Protein Engineering Platform, Molecular Interaction Service, F-75252 Paris, France.

Sophie Sayettat (S)

Institut Cochin, Inserm U1016, CNRS UMR8104, Université Paris-Cité, 75014 Paris, France.

Nassima Bekaddour (N)

CNRS UMR-8601, Université Paris Cité, 75006 Paris, France.
Chemistry and Biology, Modeling and Immunology for Therapy (CBMIT), Paris, France.

Kanika Vanshylla (K)

Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.

Franziska Kleipass (F)

Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.

Sebastian Wiese (S)

Core Unit Mass Spectrometry and Proteomics, Ulm University, 89081 Ulm, Germany.

Ludger Ständker (L)

Core Facility Functional Peptidomics, Ulm University Medical Center, 89081 Ulm, Germany.

Florian Klein (F)

Laboratory of Experimental Immunology, Institute of Virology, Faculty of Medicine and University Hospital Cologne, University of Cologne, 50931 Cologne, Germany.
German Center for Infection Research (DZIF), Partner site Bonn-Cologne, 50937 Cologne, Germany.
Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany.

Bernard Lagane (B)

Infinity, Université de Toulouse, CNRS, INSERM, Toulouse, France.

Arnaud Boonen (A)

Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Herestraat 49, P.O. Box 1030, 3000 Leuven, Belgium.

Dominique Schols (D)

Laboratory of Virology and Chemotherapy, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU Leuven, Herestraat 49, P.O. Box 1030, 3000 Leuven, Belgium.

Serge Benichou (S)

Institut Cochin, Inserm U1016, CNRS UMR8104, Université Paris-Cité, 75014 Paris, France.

Elsa Sanchez-Garcia (E)

Computational Biochemistry, Center of Medical Biotechnology, University of Duisburg-Essen, Universitätsstr. 2, 45141 Essen, Germany.
Computational Bioengineering, Department of Biochemical and Chemical Engineering, Emil-Figge Str. 66., 44227 Dortmund, Germany.

Jean-Philippe Herbeuval (JP)

CNRS UMR-8601, Université Paris Cité, 75006 Paris, France.
Chemistry and Biology, Modeling and Immunology for Therapy (CBMIT), Paris, France.

Jan Münch (J)

Institute of Molecular Virology, Ulm University Medical Center, 89081 Ulm, Germany.

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