Somatic mutational landscape of hereditary hematopoietic malignancies caused by germline variants in RUNX1, GATA2, and DDX41.


Journal

Blood advances
ISSN: 2473-9537
Titre abrégé: Blood Adv
Pays: United States
ID NLM: 101698425

Informations de publication

Date de publication:
24 10 2023
Historique:
accepted: 19 06 2023
received: 23 02 2023
medline: 23 10 2023
pubmed: 5 7 2023
entrez: 5 7 2023
Statut: ppublish

Résumé

Individuals with germ line variants associated with hereditary hematopoietic malignancies (HHMs) have a highly variable risk for leukemogenesis. Gaps in our understanding of premalignant states in HHMs have hampered efforts to design effective clinical surveillance programs, provide personalized preemptive treatments, and inform appropriate counseling for patients. We used the largest known comparative international cohort of germline RUNX1, GATA2, or DDX41 variant carriers without and with hematopoietic malignancies (HMs) to identify patterns of genetic drivers that are unique to each HHM syndrome before and after leukemogenesis. These patterns included striking heterogeneity in rates of early-onset clonal hematopoiesis (CH), with a high prevalence of CH in RUNX1 and GATA2 variant carriers who did not have malignancies (carriers-without HM). We observed a paucity of CH in DDX41 carriers-without HM. In RUNX1 carriers-without HM with CH, we detected variants in TET2, PHF6, and, most frequently, BCOR. These genes were recurrently mutated in RUNX1-driven malignancies, suggesting CH is a direct precursor to malignancy in RUNX1-driven HHMs. Leukemogenesis in RUNX1 and DDX41 carriers was often driven by second hits in RUNX1 and DDX41, respectively. This study may inform the development of HHM-specific clinical trials and gene-specific approaches to clinical monitoring. For example, trials investigating the potential benefits of monitoring DDX41 carriers-without HM for low-frequency second hits in DDX41 may now be beneficial. Similarly, trials monitoring carriers-without HM with RUNX1 germ line variants for the acquisition of somatic variants in BCOR, PHF6, and TET2 and second hits in RUNX1 are warranted.

Identifiants

pubmed: 37406166
pii: 496710
doi: 10.1182/bloodadvances.2023010045
pmc: PMC10582382
doi:

Substances chimiques

Core Binding Factor Alpha 2 Subunit 0
DEAD-box RNA Helicases EC 3.6.4.13
RUNX1 protein, human 0
GATA2 protein, human 0
GATA2 Transcription Factor 0
DDX41 protein, human EC 3.6.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

6092-6107

Informations de copyright

Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution.

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Auteurs

Claire C Homan (CC)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Michael W Drazer (MW)

Departments of Medicine and Human Genetics, Section of Hematology/Oncology, Center for Clinical Cancer Genetics, and The University of Chicago Comprehensive Cancer Center, The University of Chicago, Chicago, IL.

Kai Yu (K)

Division of Intramural Research, Oncogenesis and Development Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD.

David M Lawrence (DM)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
ACRF Genomics Facility, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, SA, Australia.

Jinghua Feng (J)

UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
ACRF Genomics Facility, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, SA, Australia.

Luis Arriola-Martinez (L)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Matthew J Pozsgai (MJ)

Departments of Medicine and Human Genetics, Section of Hematology/Oncology, Center for Clinical Cancer Genetics, and The University of Chicago Comprehensive Cancer Center, The University of Chicago, Chicago, IL.

Kelsey E McNeely (KE)

Departments of Medicine and Human Genetics, Section of Hematology/Oncology, Center for Clinical Cancer Genetics, and The University of Chicago Comprehensive Cancer Center, The University of Chicago, Chicago, IL.

Thuong Ha (T)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Parvathy Venugopal (P)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Peer Arts (P)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Sarah L King-Smith (SL)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Jesse Cheah (J)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Mark Armstrong (M)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Paul Wang (P)

UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
ACRF Genomics Facility, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, SA, Australia.

Csaba Bödör (C)

HCEMM-SE Molecular Oncohematology Research Group, 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary.

Alan B Cantor (AB)

Division of Hematology/Oncology, Boston Children's Hospital and Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA.

Mario Cazzola (M)

Department of Molecular Medicine, University of Pavia, Pavia, Italy.
Department of Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Erin Degelman (E)

Alberta Children's Hospital, Calgary, Alberta, Canada.

Courtney D DiNardo (CD)

Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.

Nicolas Duployez (N)

Laboratory of Hematology, Biology and Pathology Center, Centre Hospitalier Regional Universitaire de Lille, Lille, France.
Jean-Pierre Aubert Research Center, INSERM, Universitaire de Lille, Lille, France.

Remi Favier (R)

Assistance Publique-Hôpitaux de Paris, Armand Trousseau Children's Hospital, Paris, France.

Stefan Fröhling (S)

Department of Translational Medical Oncology, National Center for Tumor Diseases and German Cancer Research Center (DKFZ), Heidelberg, Germany.
German Cancer Consortium (DKTK), Heidelberg, Germany.

Ana Rio-Machin (A)

Centre for Haemato-Oncology, Barts Cancer Institute, Queen Mary University of London, London, United Kingdom.

Jeffery M Klco (JM)

St Jude Children's Research Hospital, Memphis, TN.

Alwin Krämer (A)

Clinical Cooperation Unit Molecular Hematology/Oncology, German Cancer Research Center (DKFZ) and Department of Internal Medicine V, University of Heidelberg, Heidelberg, Germany.

Mineo Kurokawa (M)

Department of Hematology & Oncology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Joanne Lee (J)

Department of Haematology-Oncology, National University Cancer Institute, National University Health System, Singapore.

Luca Malcovati (L)

Department of Molecular Medicine, University of Pavia, Pavia, Italy.
Department of Hematology Oncology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Neil V Morgan (NV)

Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom.

Georges Natsoulis (G)

Imago Biosciences, Inc, San Francisco, CA.

Carolyn Owen (C)

Division of Hematology and Hematological Malignancies, Foothills Medical Centre, Calgary, AB, Canada.

Keyur P Patel (KP)

Department of Leukemia, The University of Texas MD Anderson Cancer Center, Houston, TX.

Claude Preudhomme (C)

Laboratory of Hematology, Biology and Pathology Center, Centre Hospitalier Regional Universitaire de Lille, Lille, France.
Jean-Pierre Aubert Research Center, INSERM, Universitaire de Lille, Lille, France.

Hana Raslova (H)

Institut Gustave Roussy, Université Paris Sud, Equipe Labellisée par la Ligue Nationale Contre le Cancer, Villejuif, France.

Hugh Rienhoff (H)

Imago Biosciences, Inc, San Francisco, CA.

Tim Ripperger (T)

Department of Human Genetics, Hannover Medical School, Hannover, Germany.

Rachael Schulte (R)

Division of Pediatric Hematology and Oncology, Riley Children's Hospital, Indiana University School of Medicine, Indianapolis, IN.

Kiran Tawana (K)

Department of Haematology, Addenbrooke's Hospital, Cambridge, United Kingdom.

Elvira Velloso (E)

Service of Hematology, Transfusion and Cell Therapy and Laboratory of Medical Investigation in Pathogenesis and Directed Therapy in Onco-Immuno-Hematology (LIM-31) HCFMUSP, University of Sao Paulo Medical School, Sao Paulo, Brazil.
Genetics Laboratory, Hospital Israelita Albert Einstein, Sao Paulo, Brazil.

Benedict Yan (B)

Department of Haematology-Oncology, National University Cancer Institute, National University Health System, Singapore.

Erika Kim (E)

National Cancer Institute, National Institutes of Health, Rockville, MD.

Raman Sood (R)

Division of Intramural Research, Oncogenesis and Development Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD.

Amy P Hsu (AP)

National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.

Steven M Holland (SM)

National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.

Kerry Phillips (K)

Adult Genetics Unit, Royal Adelaide Hospital, Adelaide, SA, Australia.

Nicola K Poplawski (NK)

Adult Genetics Unit, Royal Adelaide Hospital, Adelaide, SA, Australia.
Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.

Milena Babic (M)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.

Andrew H Wei (AH)

Department of Haematology, Peter McCallum Cancer Centre, Royal Melbourne Hospital, Walter and Eliza Hall Institute of Medical Research, The University of Melbourne, Melbourne, VIC, Australia.

Cecily Forsyth (C)

Central Coast Haematology, North Gosford, NSW, Australia.

Helen Mar Fan (H)

Department of Medicine, The University of Queensland, Brisbane, QLD, Australia.

Ian D Lewis (ID)

Adelaide Oncology & Haematology, North Adelaide, SA, Australia.

Julian Cooney (J)

Department of Haematology, Fiona Stanley Hospital, Murdoch, WA, Australia.

Rachel Susman (R)

Genetic Health Queensland, Royal Brisbane and Women's Hospital, Brisbane, QLD, Australia.

Lucy C Fox (LC)

Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.

Piers Blombery (P)

Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.

Deepak Singhal (D)

Department of Haematology, SA Pathology, Adelaide, SA, Australia.

Devendra Hiwase (D)

Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.
Department of Haematology, SA Pathology, Adelaide, SA, Australia.

Belinda Phipson (B)

Bioinformatics Division, Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC, Australia.
Department of Paediatrics and Department of Molecular Biology, The University of Melbourne, Melbourne, VIC, Australia.

Andreas W Schreiber (AW)

UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
ACRF Genomics Facility, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, SA, Australia.
School of Biological Sciences, The University of Adelaide, Adelaide, SA, Australia.

Christopher N Hahn (CN)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.

Hamish S Scott (HS)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
ACRF Genomics Facility, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, SA, Australia.
Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.

Paul Liu (P)

Division of Intramural Research, Oncogenesis and Development Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD.

Lucy A Godley (LA)

Departments of Medicine and Human Genetics, Section of Hematology/Oncology, Center for Clinical Cancer Genetics, and The University of Chicago Comprehensive Cancer Center, The University of Chicago, Chicago, IL.

Anna L Brown (AL)

Department of Genetics and Molecular Pathology, Centre for Cancer Biology, An alliance between SA Pathology and the University of South Australia, Adelaide, Australia.
UniSA Clinical and Health Sciences, University of South Australia, Adelaide, Australia.
Adelaide Medical School, The University of Adelaide, Adelaide, SA, Australia.

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