Research Note: A novel method for preparation of egg yolk immunoglobulin Y against Porphyromonas gingivalis.
Porphyromonas gingivalis
biofilm
egg yolk immunoglobulin Y
oleic acid
therapy
Journal
Poultry science
ISSN: 1525-3171
Titre abrégé: Poult Sci
Pays: England
ID NLM: 0401150
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
05
06
2022
revised:
31
03
2023
accepted:
07
06
2023
medline:
28
8
2023
pubmed:
6
7
2023
entrez:
5
7
2023
Statut:
ppublish
Résumé
Porphyromonas gingivalis (P. gingivalis, P. g) is the main pathogen of periodontal disease, which is treated with egg yolk immunoglobulin Y (IgY) against P. gingivalis. In order to quickly obtain IgY, 30 hens were immunized with inactivated P. gingivalis. The purification of IgY was carried out by the oleic acid (OA) method and the classical method (AS), respectively. The IgY antibody characteristics and antibacterial effects in HPDLF cells were detected by SDS-PAGE, indirect ELISA, Western blot and viability/toxicity assays. SDS-PAGE and Western blot analysis showed that IgY molecules which were rapidly purified by OA method were complete and specific to P. gingivalis. In addition, the results of crystal violet staining and bacterial staining indicated that IgY could agglutinate with P. gingivalis, inhibiting bacterial invasion of host cells. This study is the first to rapidly and efficiently purify IgY by OA method, and the purified IgY is expected to be used in the detection and treatment of P. gingivalis.
Identifiants
pubmed: 37406435
pii: S0032-5791(23)00382-6
doi: 10.1016/j.psj.2023.102863
pmc: PMC10466223
pii:
doi:
Substances chimiques
IgY
0
Immunoglobulins
0
Antibodies
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102863Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
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