The role of interleukin-18 and interleukin-18 binding protein in K/BxN serum transfer-induced arthritis.

Animals Mice Interleukin-18 / genetics Endothelial Cells / metabolism Cytokines Still's Disease, Adult-Onset RNA, Messenger
K/BxN serum transfer-induced arthritis Still’s disease interleukin-18 interleukin-18 binding protein rheumatoid arthritis systemic juvenile idiopathic arthritis

Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2023
Historique:
received: 01 05 2023
accepted: 24 05 2023
medline: 10 7 2023
pubmed: 7 7 2023
entrez: 7 7 2023
Statut: epublish

Résumé

Interleukin-18 is a proinflammatory cytokine, the activity of which is regulated by its natural inhibitor, IL-18 binding protein (IL-18BP). Elevated circulating levels of IL-18 have been observed in patients with systemic juvenile idiopathic arthritis (sJIA) and adult-onset Still's disease (AOSD), two conditions associated with dysregulated innate immune responses. This study examines the expression and function of IL-18 and IL-18BP in K/BxN serum transfer arthritis (STA), a model that is uniquely dependent on innate immune responses. Naïve and serum transfer-induced arthritis (STA) wild-type (WT) mice were used to examine the articular levels of IL-18 and IL-18BP mRNA by RT-qPCR. The cellular sources of IL-18BP in the joints were determined by using IL-18 and IL-18BP mRNA levels were significantly increased in arthritic as compared to normal joints. Synovial neutrophils, macrophages, and endothelial cells represented the cellular sources of IL-18BP in arthritic joints, whereas IL-18BP production was limited to endothelial cells in non-inflamed joints. The incidence and severity of arthritis were similar in IL-18BP KO and IL-18 KO compared to their WT littermates. Transcript levels of different inflammatory cytokines were not different in the two KO mouse lines compared to WT mice. Although IL-18 and IL-18BP levels were increased in arthritic joints, our results show that the IL-18/IL-18BP balance is not involved in the regulation of STA.

Sections du résumé

Background
Interleukin-18 is a proinflammatory cytokine, the activity of which is regulated by its natural inhibitor, IL-18 binding protein (IL-18BP). Elevated circulating levels of IL-18 have been observed in patients with systemic juvenile idiopathic arthritis (sJIA) and adult-onset Still's disease (AOSD), two conditions associated with dysregulated innate immune responses. This study examines the expression and function of IL-18 and IL-18BP in K/BxN serum transfer arthritis (STA), a model that is uniquely dependent on innate immune responses.
Methods
Naïve and serum transfer-induced arthritis (STA) wild-type (WT) mice were used to examine the articular levels of IL-18 and IL-18BP mRNA by RT-qPCR. The cellular sources of IL-18BP in the joints were determined by using
Results
IL-18 and IL-18BP mRNA levels were significantly increased in arthritic as compared to normal joints. Synovial neutrophils, macrophages, and endothelial cells represented the cellular sources of IL-18BP in arthritic joints, whereas IL-18BP production was limited to endothelial cells in non-inflamed joints. The incidence and severity of arthritis were similar in IL-18BP KO and IL-18 KO compared to their WT littermates. Transcript levels of different inflammatory cytokines were not different in the two KO mouse lines compared to WT mice.
Conclusion
Although IL-18 and IL-18BP levels were increased in arthritic joints, our results show that the IL-18/IL-18BP balance is not involved in the regulation of STA.

Identifiants

DOI: 10.3389/fimmu.2023.1215364 PMID: 37415987 PMC: PMC10320286
pubmed: 37415987
doi: 10.3389/fimmu.2023.1215364
pmc: PMC10320286
doi:

Substances chimiques

Interleukin-18 0
interleukin-18 binding protein 0
Cytokines 0
RNA, Messenger 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1215364

Informations de copyright

Copyright © 2023 Fauteux-Daniel, Merlo Pich, Girard-Guyonvarc’h, Caruso, Rodriguez and Gabay.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

J Rheumatol. 2004 Nov;31(11):2189-98
pubmed: 15517632
Arthritis Res Ther. 2014 Apr 24;16(2):R102
pubmed: 24762050
Immunity. 1999 Jan;10(1):127-36
pubmed: 10023777
Rheumatology (Oxford). 2003 Mar;42(3):442-5
pubmed: 12626794
Infect Immun. 1989 Feb;57(2):590-5
pubmed: 2492265
Cytokine. 2022 Feb;150:155781
pubmed: 34923222
Rheumatology (Oxford). 2020 Feb 1;59(2):442-445
pubmed: 31359055
Ann Rheum Dis. 2010 Jan;69(1):276-83
pubmed: 19188194
Arthritis Rheum. 2003 Feb;48(2):339-47
pubmed: 12571842
J Clin Invest. 1999 Nov;104(10):1393-401
pubmed: 10562301
J Immunol. 2003 Feb 15;170(4):2100-5
pubmed: 12574381
Immunity. 1998 Mar;8(3):383-90
pubmed: 9529155
J Rheumatol. 2001 Aug;28(8):1779-87
pubmed: 11508579
J Rheumatol. 2007 Apr;34(4):823-30
pubmed: 17343316
Curr Opin Allergy Clin Immunol. 2017 Dec;17(6):398-404
pubmed: 28957823
Curr Protoc Immunol. 2008 May;Chapter 15:15.22.1-15.22.12
pubmed: 18491295
J Exp Med. 2002 Jul 1;196(1):77-85
pubmed: 12093872
J Immunol. 2017 Apr 1;198(7):2916-2926
pubmed: 28235865
J Immunol. 2001 Jan 1;166(1):517-21
pubmed: 11123331
Gene Ther. 2003 Jun;10(12):1004-11
pubmed: 12776157
Eur Cytokine Netw. 2010 Dec;21(4):264-71
pubmed: 21126942
J Clin Invest. 2001 Dec;108(12):1825-32
pubmed: 11748266
Arthritis Rheumatol. 2017 Feb;69(2):447-459
pubmed: 27788288
J Biol Chem. 1997 Oct 17;272(42):26595-603
pubmed: 9334240
Arthritis Rheum. 2001 Feb;44(2):275-85
pubmed: 11229457
Rheumatology (Oxford). 2016 Dec;55(12):2237-2247
pubmed: 27616144
Arthritis Res Ther. 2013 Jan 16;15(1):R13
pubmed: 23324173
J Exp Med. 2020 May 4;217(5):
pubmed: 32097462
J Immunol. 2020 Aug 15;205(4):1167-1175
pubmed: 32651219
J Immunol. 2000 Jun 15;164(12):6495-502
pubmed: 10843707
Arthritis Rheum. 2010 Mar;62(3):722-31
pubmed: 20131228
Clin Rheumatol. 2006 Jul;25(4):448-52
pubmed: 16362448
J Immunol. 2001 Dec 15;167(12):7038-43
pubmed: 11739524
J Immunol. 2023 Jun 1;210(11):1790-1803
pubmed: 37074208
J Immunol. 2000 Dec 1;165(11):6553-8
pubmed: 11086098
Blood. 2018 Mar 29;131(13):1442-1455
pubmed: 29326099
Ann Rheum Dis. 2002 Aug;61(8):726-9
pubmed: 12117681
Immunol Cell Biol. 2006 Apr;84(2):166-73
pubmed: 16519734
Ann Rheum Dis. 2018 Jun;77(6):840-847
pubmed: 29472362
Cells. 2019 Dec 18;9(1):
pubmed: 31861496

Auteurs

Sebastien Fauteux-Daniel (S)

Division of Rheumatology, Department of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Department of Pathology and Immunology, University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Geneva Centre for Inflammation Research, Geneva, Switzerland.

Laura M Merlo Pich (LM)

Division of Rheumatology, Department of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Department of Pathology and Immunology, University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Geneva Centre for Inflammation Research, Geneva, Switzerland.

Charlotte Girard-Guyonvarc'h (C)

Division of Rheumatology, Department of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Department of Pathology and Immunology, University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Geneva Centre for Inflammation Research, Geneva, Switzerland.

Assunta Caruso (A)

Division of Rheumatology, Department of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Department of Pathology and Immunology, University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Geneva Centre for Inflammation Research, Geneva, Switzerland.

Emiliana Rodriguez (E)

Division of Rheumatology, Department of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Department of Pathology and Immunology, University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Geneva Centre for Inflammation Research, Geneva, Switzerland.

Cem Gabay (C)

Division of Rheumatology, Department of Medicine, Geneva University Hospitals, Geneva, Switzerland.
Department of Pathology and Immunology, University of Geneva, Faculty of Medicine, Geneva, Switzerland.
Geneva Centre for Inflammation Research, Geneva, Switzerland.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The role of interleukin-18 and interleukin-18...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › The role of interleukin-18 and interleukin-18...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interleukines › Interleukine-18 › The role of interleukin-18 and interleukin-18...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › The role of interleukin-18 and interleukin-18...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › The role of interleukin-18 and interleukin-18...
questionsmedicales.fr › Maladies du système immunitaire › Maladies auto-immunes › Polyarthrite rhumatoïde › Maladie de Still débutant à l'âge adulte › The role of interleukin-18 and interleukin-18...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › The role of interleukin-18 and interleukin-18...