5'/3' RACE method for sequencing the 5' and 3' untranslated regions of Zika virus.


Journal

Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870

Informations de publication

Date de publication:
10 Jul 2023
Historique:
received: 12 09 2022
accepted: 19 05 2023
medline: 12 7 2023
pubmed: 10 7 2023
entrez: 10 7 2023
Statut: epublish

Résumé

The spread of Zika virus (ZIKV) from the African continent to the Americas promoted its molecular evolution, as reflected by mutations in its RNA genome. Most of the ZIKV genome sequences in the GenBank database have incomplete 5' and 3' UTR sequences, reflecting the deficiency of whole-genome sequencing technologies to resolve the sequences of the genome ends. We modified a protocol for rapid amplification of cDNA ends (RACE) to determine the complete sequences of the 5' and 3' UTRs of a previously reported ZIKV isolate (GenBank no. MH544701.1). This strategy is useful for determining 5' and 3' UTR sequences of ZIKV isolates and will be useful for comparative genomics applications.

Identifiants

pubmed: 37428234
doi: 10.1007/s00705-023-05820-8
pii: 10.1007/s00705-023-05820-8
doi:

Substances chimiques

3' Untranslated Regions 0
RNA, Viral 0
5' Untranslated Regions 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

204

Subventions

Organisme : Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS)
ID : 210474455818

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

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Auteurs

Diego Alejandro Álvarez-Díaz (DA)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
Grupo de Genómica de Microorganismos Emergentes, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

José Aldemar Usme-Ciro (JA)

Centro de Investigación en Salud para el Trópico-CIST, Universidad Cooperativa de Colombia, Santa Marta, Colombia.

Sheryll Corchuelo (S)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Julián Ricardo Naizaque (JR)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Jorge Alonso Rivera (JA)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Hernán Darío Castiblanco-Martínez (HD)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Orlando Torres-Fernández (O)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Aura Caterine Rengifo (AC)

Grupo de Morfología Celular, Dirección de Investigación en Salud Pública, Instituto Nacional de Salud, Bogotá, D.C., Colombia. arengifo@ins.gov.co.

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