Lustro: High-Throughput Optogenetic Experiments Enabled by Automation and a Yeast Optogenetic Toolkit.
MoClo
automation
high throughput
optogenetics
synthetic transcription factors
yeast
Journal
ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075
Informations de publication
Date de publication:
21 07 2023
21 07 2023
Historique:
pmc-release:
11
07
2024
medline:
27
7
2023
pubmed:
12
7
2023
entrez:
11
7
2023
Statut:
ppublish
Résumé
Optogenetic systems use genetically encoded light-sensitive proteins to control cellular processes. This provides the potential to orthogonally control cells with light; however, these systems require many design-build-test cycles to achieve a functional design and multiple illumination variables need to be laboriously tuned for optimal stimulation. We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in
Identifiants
pubmed: 37434272
doi: 10.1021/acssynbio.3c00215
pmc: PMC10368012
doi:
Substances chimiques
Transcription Factors
0
Types de publication
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Letter
Langues
eng
Sous-ensembles de citation
IM
Pagination
1943-1951Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM128873
Pays : United States
Organisme : NHGRI NIH HHS
ID : T32 HG002760
Pays : United States
Commentaires et corrections
Type : UpdateOf
Type : ErratumIn
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