Isolation and genome characterization of Lloviu virus from Italian Schreibers's bats.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 07 2023
13 07 2023
Historique:
received:
18
04
2023
accepted:
06
07
2023
medline:
17
7
2023
pubmed:
14
7
2023
entrez:
13
7
2023
Statut:
epublish
Résumé
Lloviu cuevavirus (LLOV) was the first identified member of Filoviridae family outside the Ebola and Marburgvirus genera. A massive die-off of Schreibers's bats (Miniopterus schreibersii) in the Iberian Peninsula in 2002 led to its initial discovery. Recent studies with recombinant and wild-type LLOV isolates confirmed the zoonotic nature of the virus in vitro. We examined bat samples from Italy for the presence of LLOV in an area outside of the currently known distribution range of the virus. We detected one positive sample from 2020, sequenced the complete coding region of the viral genome and established an infectious isolate of the virus. In addition, we performed the first comprehensive evolutionary analysis of the virus, using the Spanish, Hungarian and the Italian sequences. The most important achievement of this study is the establishment of an additional infectious LLOV isolate from a bat sample using the SuBK12-08 cells, demonstrating that this cell line is highly susceptible to LLOV infection and confirming the previous observation that these bats are effective hosts of the virus in nature. This result further strengthens the role of bats as the natural hosts for zoonotic filoviruses.
Identifiants
pubmed: 37443182
doi: 10.1038/s41598-023-38364-7
pii: 10.1038/s41598-023-38364-7
pmc: PMC10344946
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11310Subventions
Organisme : NIAID NIH HHS
ID : R21 AI169646
Pays : United States
Informations de copyright
© 2023. The Author(s).
Références
Emerg Microbes Infect. 2018 Apr 18;7(1):66
pubmed: 29670087
Genome Biol. 2014;15(11):540
pubmed: 25416632
PLoS Pathog. 2011 Oct;7(10):e1002304
pubmed: 22039362
FEMS Microbiol Rev. 2016 Jul;40(4):494-519
pubmed: 27268907
Nature. 2018 Apr;556(7700):197-202
pubmed: 29618816
J Infect Dis. 2011 Nov;204 Suppl 3:S757-60
pubmed: 21987747
PLoS Pathog. 2009 Jul;5(7):e1000536
pubmed: 19649327
Clin Lab Med. 2010 Mar;30(1):161-77
pubmed: 20513546
Bioinformatics. 2018 Aug 1;34(15):2666-2669
pubmed: 29547981
Lancet. 2019 Mar 2;393(10174):936-948
pubmed: 30777297
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027
pubmed: 33892491
Nat Commun. 2022 Mar 31;13(1):1706
pubmed: 35361761
Bioinformatics. 2018 Sep 15;34(18):3094-3100
pubmed: 29750242
J Infect Dis. 2015 Oct 1;212 Suppl 2:S101-8
pubmed: 25786916
Viruses. 2019 Apr 19;11(4):
pubmed: 31010201
Integr Zool. 2022 Jan;17(1):2-23
pubmed: 34003577
J Infect Dis. 2018 Nov 22;218(suppl_5):S698-S704
pubmed: 30321368
Nat Commun. 2020 Jan 24;11(1):510
pubmed: 31980636
J Infect Dis. 2011 Nov;204 Suppl 3:S1011-20
pubmed: 21987737
Emerg Infect Dis. 2019 Sep;25(9):
pubmed: 31310231
Viruses. 2015 Jun 25;7(7):3420-42
pubmed: 26120867
PLoS Pathog. 2022 Feb 4;18(2):e1010268
pubmed: 35120176
Ecohealth. 2022 Mar;19(1):5-7
pubmed: 35107640
Nat Commun. 2017 Feb 13;8:14446
pubmed: 28194016
Gigascience. 2021 Feb 16;10(2):
pubmed: 33590861
Viruses. 2021 Oct 14;13(10):
pubmed: 34696493
One Health. 2023 Jan 13;16:100488
pubmed: 36691393
BMC Bioinformatics. 2004 Aug 19;5:113
pubmed: 15318951
Curr Opin Pharmacol. 2021 Oct;60:268-274
pubmed: 34482213
J Virol. 2014 Jan;88(1):99-109
pubmed: 24131711
Microbiol Aust. 2017 Mar;38(1):12-16
pubmed: 28603449
Emerg Infect Dis. 2021 Dec;27(12):3082-3091
pubmed: 34808081
Nat Microbiol. 2019 Mar;4(3):390-395
pubmed: 30617348
Cell Rep. 2020 Jan 14;30(2):308-319.e5
pubmed: 31940478
Nat Microbiol. 2018 Oct;3(10):1084-1089
pubmed: 30150734
Sci Rep. 2020 Feb 3;10(1):1740
pubmed: 32015375