Humanization of the Reaction Specificity of Mouse Alox15b Inversely Modified the Susceptibility of Corresponding Knock-In Mice in Two Different Animal Inflammation Models.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
03 Jul 2023
Historique:
received: 05 05 2023
revised: 28 06 2023
accepted: 29 06 2023
medline: 17 7 2023
pubmed: 14 7 2023
entrez: 14 7 2023
Statut: epublish

Résumé

Mammalian arachidonic acid lipoxygenases (ALOXs) have been implicated in the pathogenesis of inflammatory diseases, and its pro- and anti-inflammatory effects have been reported for different ALOX-isoforms. Human ALOX15B oxygenates arachidonic acid to its 15-hydroperoxy derivative, whereas the corresponding 8-hydroperoxide is formed by mouse Alox15b (Alox8). This functional difference impacts the biosynthetic capacity of the two enzymes for creating pro- and anti-inflammatory eicosanoids. To explore the functional consequences of the humanization of the reaction specificity of mouse

Identifiants

pubmed: 37446212
pii: ijms241311034
doi: 10.3390/ijms241311034
pmc: PMC10341735
pii:
doi:

Substances chimiques

Arachidonic Acid 27YG812J1I
sodium sulfate 0YPR65R21J
Dextrans 0
Anti-Inflammatory Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : HE8295/1-1
Organisme : Deutsche Forschungsgemeinschaft
ID : KU961/13-1
Organisme : Deutsche Forschungsgemeinschaft
ID : KU961/14-1

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Marjann Schäfer (M)

Department of Biochemistry, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
Institute for Nutritional Sciences, University Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany.

Florian Reisch (F)

Department of Biochemistry, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, D-10117 Berlin, Germany.
Institute for Nutritional Sciences, University Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany.

Dominika Labuz (D)

Department of Experimental Anesthesiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, D-12203 Berlin, Germany.

Halina Machelska (H)

Department of Experimental Anesthesiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, D-12203 Berlin, Germany.

Sabine Stehling (S)

Department of Biochemistry, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, D-10117 Berlin, Germany.

Gerhard P Püschel (GP)

Institute for Nutritional Sciences, University Potsdam, Arthur-Scheunert-Allee 114-116, D-14558 Nuthetal, Germany.

Michael Rothe (M)

Lipidomix GmbH, Robert-Roessle-Straße 10, D-13125 Berlin, Germany.

Dagmar Heydeck (D)

Department of Biochemistry, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, D-10117 Berlin, Germany.

Hartmut Kuhn (H)

Department of Biochemistry, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Charitéplatz 1, D-10117 Berlin, Germany.

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Classifications MeSH