Determination of the Main Phase Transition Temperature of Phospholipids by Oscillatory Rheology.
complex viscosity
oscillatory rheology
phase transition temperature
phospholipid
reverse-type phospholipid complex
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
29 Jun 2023
29 Jun 2023
Historique:
received:
13
06
2023
revised:
22
06
2023
accepted:
27
06
2023
medline:
17
7
2023
pubmed:
14
7
2023
entrez:
14
7
2023
Statut:
epublish
Résumé
Knowledge of the physical and chemical properties of phospholipids, such as phase transition temperatures (Tc), is of great importance in order to reveal the functionalities of biological and artificial membranes. Our research group developed an oscillatory rheological method for the simple and rapid determination of phase transition temperatures (Tc). The phospholipids constructing the membranes undergo conformational changes at their Tc, which cause alterations of viscoelastic properties of the molecules. The oscillatory technique recommended by us proved to be appropriate to reveal the altered molecular properties of phospholipids as tracking the slightest changes in the viscoelasticity. Our study demonstrates the abrupt changes in rheological properties at Tc for the following phospholipids: 1,2-Dimyristoyl-sn-glycero-3-Phosphocholine (DMPC), 1,2-Dipalmitoyl-sn-glycero-3-Phosphatidylcholine (DPPC), and 1,2-Distearoyl-sn-glycero-3-Phosphocholine (DSPC), proving that the applied methodology is adequate for determining the Tc of phospholipids.
Identifiants
pubmed: 37446784
pii: molecules28135125
doi: 10.3390/molecules28135125
pmc: PMC10343600
pii:
doi:
Substances chimiques
Phospholipids
0
Lipid Bilayers
0
1,2-Dipalmitoylphosphatidylcholine
2644-64-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Adv Drug Deliv Rev. 2022 Sep;188:114416
pubmed: 35787388
Biophys J. 2004 Jun;86(6):3783-93
pubmed: 15189874
Pharmaceutics. 2023 Apr 03;15(4):
pubmed: 37111613
Sci Rep. 2018 Oct 4;8(1):14815
pubmed: 30287903
Phys Rev Lett. 2002 Dec 9;89(24):246103
pubmed: 12484960
J Biochem Biophys Methods. 1990 Apr-May;20(4):353-6
pubmed: 2365951
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862-6
pubmed: 1069270
Biophys J. 2000 Oct;79(4):2056-65
pubmed: 11023909
Biochemistry. 1977 Nov 15;16(23):5100-8
pubmed: 911814
Biophys J. 2005 Aug;89(2):1094-101
pubmed: 15879467
Chem Phys Lipids. 2006 Jan;139(1):11-9
pubmed: 16253216
Ultramicroscopy. 2003 Oct-Nov;97(1-4):217-27
pubmed: 12801674
Sensors (Basel). 2012;12(7):9530-50
pubmed: 23012557
Drug Dev Ind Pharm. 2013 Feb;39(2):197-204
pubmed: 22443684
Biochemistry. 1978 Oct 17;17(21):4475-80
pubmed: 581473
Chem Phys Lipids. 1992 Nov;63(1-2):139-48
pubmed: 1486656