ZBTB40 is a telomere-associated protein and protects telomeres in human ALT cells.
ALT
APBs
DNA damage
ZBTB40
telomere
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
22
10
2022
revised:
05
07
2023
accepted:
06
07
2023
medline:
2
10
2023
pubmed:
17
7
2023
entrez:
16
7
2023
Statut:
ppublish
Résumé
Alternative lengthening of telomeres (ALTs) mechanism is activated in some somatic, germ cells, and human cancer cells. However, the key regulators and mechanisms of the ALT pathway remain elusive. Here we demonstrated that ZBTB40 is a novel telomere-associated protein and binds to telomeric dsDNA through its N-terminal BTB (BR-C, ttk and bab) or POZ (Pox virus and Zinc finger) domain in ALT cells. Notably, the knockout or knockdown of ZBTB40 resulted in the telomere dysfunction-induced foci and telomere lengthening in the ALT cells. The results also show that ZBTB40 is associated with ALT-associated promyelocytic leukemia nuclear bodies, and the loss of ZBTB40 induces the accumulation of the ALT-associated promyelocytic leukemia nuclear bodies in U2OS cells. Taken together, our results implicate that ZBTB40 is a key player of telomere protection and telomere lengthening regulation in human ALT cells.
Identifiants
pubmed: 37454741
pii: S0021-9258(23)02081-1
doi: 10.1016/j.jbc.2023.105053
pmc: PMC10480536
pii:
doi:
Substances chimiques
ZBTB40 protein, human
0
DNA
9007-49-2
DNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105053Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.