Photoinduced bidirectional switching in lipid membranes containing azobenzene glycolipids.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
16 Jul 2023
Historique:
received: 02 11 2022
accepted: 06 07 2023
medline: 17 7 2023
pubmed: 17 7 2023
entrez: 16 7 2023
Statut: epublish

Résumé

Following the reaction of biological membranes to external stimuli reveals fundamental insights into cellular function. Here, self-assembled lipid monolayers act as model membranes containing photoswitchable azobenzene glycolipids for investigating structural response during isomerization by combining Langmuir isotherms with X-ray scattering. Controlled in-situ trans/cis photoswitching of the azobenzene N = N double bond alters the DPPC monolayer structure, causing reproducible changes in surface pressure and layer thickness, indicating monolayer reorientation. Interestingly, for monolayers containing azobenzene glycolipids, along with the expected DPPC phase transitions an additional discontinuity is observed. The associated reorintation represents a crossover point, with the surface pressure and layer thickness changing in opposite directions above and below. This is evidence that the azobenzene glycolipids themselves change orientation within the monolayer. Such behaviour suggests that azobenzene glycolipids can act as a bidirectional switch in DPPC monolayers providing a tool to investigate membrane structure-function relationships in depth.

Identifiants

pubmed: 37455299
doi: 10.1038/s41598-023-38336-x
pii: 10.1038/s41598-023-38336-x
pmc: PMC10350456
doi:

Substances chimiques

Azo Compounds 0
azobenzene F0U1H6UG5C
Glycolipids 0
Membrane Lipids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

11480

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : SFB677
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB677
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB677
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB677
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB677
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB677
Organisme : Bundesministerium für Bildung und Forschung
ID : 05K19FK2
Organisme : Bundesministerium für Bildung und Forschung
ID : 05K19FK2
Organisme : Bundesministerium für Bildung und Forschung
ID : 05K19FK2
Organisme : Deutsches Elektronen-Synchrotron
ID : CMWS - Startup grant

Informations de copyright

© 2023. The Author(s).

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Auteurs

Jonas E Warias (JE)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.

Franziska Reise (F)

Otto Diels Institute of Organic Chemistry, Kiel University, Otto-Hahn-Platz 3-4, 24118, Kiel, Germany.

Svenja C Hövelmann (SC)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.
Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany.
Ruprecht Haensel Laboratory, Kiel University, 24118, Kiel, Germany.

Rajendra P Giri (RP)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.
Ruprecht Haensel Laboratory, Kiel University, 24118, Kiel, Germany.

Michael Röhrl (M)

Otto Diels Institute of Organic Chemistry, Kiel University, Otto-Hahn-Platz 3-4, 24118, Kiel, Germany.

Jule Kuhn (J)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.

Malte Jacobsen (M)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.

Kuntal Chatterjee (K)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Barkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA.

Thomas Arnold (T)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot, OX11 ODE, UK.
ISIS Neutron and Muon Source, Rutherford Appleton Laboratory, Didcot, OX11 0QX, UK.
Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK.
European Spallation Source ERIC, P.O Box 176, 221 00, Lund, Sweden.

Chen Shen (C)

Deutsches Elektronen-Synchrotron DESY, Notkestraße 85, 22607, Hamburg, Germany.

Sven Festersen (S)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.

Andrea Sartori (A)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.
ESRF-The European Synchrotron, 38043, Grenoble, France.

Philipp Jordt (P)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.

Olaf M Magnussen (OM)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany.
Ruprecht Haensel Laboratory, Kiel University, 24118, Kiel, Germany.

Thisbe K Lindhorst (TK)

Otto Diels Institute of Organic Chemistry, Kiel University, Otto-Hahn-Platz 3-4, 24118, Kiel, Germany.

Bridget M Murphy (BM)

Institute of Experimental and Applied Physics, Kiel University, Leibnizstr. 19, 24118, Kiel, Germany. murphy@physik.uni-kiel.de.
Ruprecht Haensel Laboratory, Kiel University, 24118, Kiel, Germany. murphy@physik.uni-kiel.de.

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Classifications MeSH