In-depth metataxonomic investigation reveals low richness, high intervariability, and diverse phylotype candidates of archaea in the human urogenital tract.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 07 2023
20 07 2023
Historique:
received:
29
12
2022
accepted:
13
07
2023
medline:
24
7
2023
pubmed:
21
7
2023
entrez:
20
7
2023
Statut:
epublish
Résumé
The urogenital microbiota is the potential principal factor in the pathophysiology of urinary tract infection and the protection of urinary tract health. Little is known about the urogenital archaeome although several reports have indicated that the archaeomes of various regions of the human body are associated with health. Accordingly, we aimed to determine the presence and diversity of archaeomes in the human urogenital tract. To explore the urogenital archaeome, voided urine specimens from 373 asymptomatic Korean individuals were used. No difference was observed in body mass index, age, or gender, according to presence of archaea. Analysis of archaeal 16S rRNA gene amplicons of archaea positive samples consisted of simple community structures, including diverse archaea, such as the phyla Methanobacteriota, Thermoproteota, and Halobacteriota. Asymptomatic individuals showed high participant-dependent intervariability in their urogenital archaeomes. The mean relative archaeal abundance was estimated to be 0.89%, and fluorescence in situ hybridisation micrographs provided evidence of archaeal cells in the human urogenital tract. In addition, the urogenital archaeome shared partial taxonomic compositional characteristics with those of the other body sites. In this study, Methanobacteriota, Thermoproteota, and Halobacteriota were suggested as inhabitants of the human urogenital tract, and a distinct human urogenital archaeome was characterised. These findings expand our knowledge of archaea-host associations in the human urogenital tract and may lead to novel insights into the role of archaea in urinary tract health.
Identifiants
pubmed: 37474649
doi: 10.1038/s41598-023-38710-9
pii: 10.1038/s41598-023-38710-9
pmc: PMC10359320
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11746Informations de copyright
© 2023. The Author(s).
Références
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
EBioMedicine. 2019 May;43:333-337
pubmed: 31072770
Nucleic Acids Res. 2022 Jan 7;50(D1):D20-D26
pubmed: 34850941
Sci Rep. 2017 Jun 22;7(1):4039
pubmed: 28642547
Microbiome. 2015 Oct 12;3:44
pubmed: 26455879
FEMS Microbiol Lett. 2012 May;330(1):1-9
pubmed: 22339687
Nat Commun. 2018 Apr 19;9(1):1557
pubmed: 29674608
PLoS One. 2010 Nov 24;5(11):e14116
pubmed: 21124791
Appl Environ Microbiol. 2006 Apr;72(4):2765-74
pubmed: 16597981
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
pubmed: 7984417
J Mol Evol. 1980 Dec;16(2):111-20
pubmed: 7463489
PLoS Comput Biol. 2009 Apr;5(4):e1000352
pubmed: 19360128
BMC Microbiol. 2011 Nov 02;11:244
pubmed: 22047020
Nat Microbiol. 2022 Jan;7(1):10-11
pubmed: 34969980
J Oral Microbiol. 2011 Feb 23;3:
pubmed: 21541092
Gastroenterology. 2007 Jul;133(1):24-33
pubmed: 17631127
PLoS One. 2013 Jun 12;8(6):e65388
pubmed: 23776475
Nucleic Acids Res. 2015 Jan;43(Database issue):D593-8
pubmed: 25414355
Front Public Health. 2019 Mar 04;7:38
pubmed: 30886840
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Nat Microbiol. 2016 Apr 04;1(6):16035
pubmed: 27572832
J Clin Microbiol. 2012 Apr;50(4):1376-83
pubmed: 22278835
Ann Transl Med. 2017 Jan;5(2):31
pubmed: 28217696
Environ Microbiol. 2001 Dec;3(12):743-54
pubmed: 11846768
J Bacteriol. 2018 Mar 12;200(7):
pubmed: 29378882
PLoS One. 2011;6(5):e19709
pubmed: 21603636
PLoS One. 2019 Feb 5;14(2):e0210813
pubmed: 30721243
J Gerontol A Biol Sci Med Sci. 2020 Sep 25;75(10):1834-1837
pubmed: 31802114
Cell Rep Med. 2022 Oct 18;3(10):100753
pubmed: 36182683
J Transl Med. 2012 Aug 28;10:174
pubmed: 22929533
Syst Appl Microbiol. 1999 Sep;22(3):434-44
pubmed: 10553296
Urology. 2016 Jun;92:26-32
pubmed: 26970449
Methods Mol Biol. 2002;179:29-42
pubmed: 11692872
World J Gastroenterol. 2009 Dec 21;15(47):5936-45
pubmed: 20014457
mBio. 2017 Nov 14;8(6):
pubmed: 29138298
Mol Biol Evol. 1987 Jul;4(4):406-25
pubmed: 3447015
Nephron. 1996;74(1):1-10
pubmed: 8883013
Gastroenterology. 2020 Oct;159(4):1459-1470.e5
pubmed: 32569776
Cytometry. 1993;14(2):136-43
pubmed: 7679962
Sci Rep. 2018 Aug 14;8(1):12157
pubmed: 30108246
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
mBio. 2014 Jul 08;5(4):e01283-14
pubmed: 25006228
Nucleic Acids Res. 2011 Jan;39(Database issue):D19-21
pubmed: 21062823
Genes Dis. 2020 Oct 13;8(6):781-797
pubmed: 34522708
Front Microbiol. 2019 Dec 05;10:2796
pubmed: 31866971
Postgrad Med J. 2005 Feb;81(952):83-6
pubmed: 15701738
FEMS Microbiol Ecol. 2000 Oct 1;34(1):45-56
pubmed: 11053735
Clin Rev Allergy Immunol. 2019 Dec;57(3):456-466
pubmed: 31522353
Microb Pathog. 2018 Apr;117:285-289
pubmed: 29477743
Mucosal Immunol. 2021 Jul;14(4):779-792
pubmed: 33542492
FEMS Microbiol Rev. 2015 Sep;39(5):631-48
pubmed: 25907112
J Pediatr Urol. 2021 Aug;17(4):545.e1-545.e8
pubmed: 34053859
Nucleic Acids Res. 2016 Jan 4;44(D1):D67-72
pubmed: 26590407
mSystems. 2021 Aug 31;6(4):e0137120
pubmed: 34282932
Front Microbiol. 2020 Oct 16;11:513305
pubmed: 33178140
Microbiome. 2020 Aug 4;8(1):114
pubmed: 32753050
Nat Biotechnol. 2019 Aug;37(8):852-857
pubmed: 31341288
PLoS One. 2013 Sep 04;8(9):e73076
pubmed: 24023812