Nicotinamide mononucleotide (NMN) alleviates the poly(I:C)-induced inflammatory response in human primary cell cultures.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
20 07 2023
Historique:
received: 31 10 2022
accepted: 14 07 2023
medline: 24 7 2023
pubmed: 21 7 2023
entrez: 20 7 2023
Statut: epublish

Résumé

NMN is the direct precursor of nicotinamide adenine dinucleotide (NAD+) and is considered as a key factor for increasing NAD+ levels and mitochondrial activity in cells. In this study, based on transcriptome analysis, we showed that NMN alleviates the poly(I:C)-induced inflammatory response in cultures of two types of human primary cells, human pulmonary microvascular endothelial cells (HPMECs) and human coronary artery endothelial cells (HCAECs). Major inflammatory mediators, including IL6 and PARP family members, were grouped into coexpressed gene modules and significantly downregulated under NMN exposure in poly(I:C)-activated conditions in both cell types. The Bayesian network analysis of module hub genes predicted common genes, including eukaryotic translation initiation factor 4B (EIF4B), and distinct genes, such as platelet-derived growth factor binding molecules, in HCAECs, which potentially regulate the identified inflammation modules. These results suggest a robust regulatory mechanism by which NMN alleviates inflammatory pathway activation, which may open up the possibility of a new role for NMN replenishment in the treatment of chronic or acute inflammation.

Identifiants

pubmed: 37474783
doi: 10.1038/s41598-023-38762-x
pii: 10.1038/s41598-023-38762-x
pmc: PMC10359400
doi:

Substances chimiques

Nicotinamide Mononucleotide 1094-61-7
NAD 0U46U6E8UK

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

11765

Informations de copyright

© 2023. The Author(s).

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Auteurs

Hitomi Sano (H)

The Systems Biology Institute, Saisei Ikedayama Bldg., 5-10-25, Higashi Gotanda, Shinagawa-ku, Tokyo, 141-0022, Japan.

Anton Kratz (A)

The Systems Biology Institute, Saisei Ikedayama Bldg., 5-10-25, Higashi Gotanda, Shinagawa-ku, Tokyo, 141-0022, Japan.

Taiko Nishino (T)

The Systems Biology Institute, Saisei Ikedayama Bldg., 5-10-25, Higashi Gotanda, Shinagawa-ku, Tokyo, 141-0022, Japan.

Haruna Imamura (H)

The Systems Biology Institute, Saisei Ikedayama Bldg., 5-10-25, Higashi Gotanda, Shinagawa-ku, Tokyo, 141-0022, Japan.

Yuki Yoshida (Y)

Ginza Research Center, Mirailab Bioscience Inc., 6F Prairie Ginza Bldg., 1-14-4, Ginza, Chuo-ku, Tokyo, 104-0061, Japan.

Noriaki Shimizu (N)

Ginza Research Center, Mirailab Bioscience Inc., 6F Prairie Ginza Bldg., 1-14-4, Ginza, Chuo-ku, Tokyo, 104-0061, Japan.

Hiroaki Kitano (H)

The Systems Biology Institute, Saisei Ikedayama Bldg., 5-10-25, Higashi Gotanda, Shinagawa-ku, Tokyo, 141-0022, Japan.

Ayako Yachie (A)

The Systems Biology Institute, Saisei Ikedayama Bldg., 5-10-25, Higashi Gotanda, Shinagawa-ku, Tokyo, 141-0022, Japan. yachie@sbi.jp.
SBX BioSciences, Inc., 1600 - 925 West Georgia Street, Vancouver, BC, V6C 3L2, Canada. yachie@sbi.jp.

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