Optimized extraction and analysis methods using liquid chromatography-tandem mass spectrometry for zearalenone and metabolites in human placental tissue.
Biomarker
Mycoestrogens
Mycotoxins
Placenta
Solid-phase extraction
Zeranols
Journal
Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
19
04
2023
revised:
31
05
2023
accepted:
01
06
2023
medline:
24
7
2023
pubmed:
24
7
2023
entrez:
24
7
2023
Statut:
epublish
Résumé
Zearalenone and its metabolites, a group of endocrine disrupting mycotoxins, have been linked to adverse reproductive health effects. They cross the placental barrier, potentially reaching the fetus. In this study, we adapted and optimized our protocol previously used for urine, to measure these mycotoxins in human placentas. We combined a supported liquid extraction step using Chem Elut cartridges with solid phase extraction on Discovery® DSC-NH2 tubes. The optimized extraction efficiencies were between 68 and 80% for all metabolites. Analysis was performed by UHPLC-HRMS using a Betasil™ Phenyl-Hexyl column eluted with a gradient of acetonitrile-methanol-water. The chromatography method separated all analytes in under 15 min. Validation experiments confirmed the method's sensitivity, with LODs ranging from 0.0055 to 0.011 pg/mg tissue. The method was linear over a range of 0.0025-1.5 pg/mg tissue with R
Identifiants
pubmed: 37484340
doi: 10.1016/j.heliyon.2023.e16940
pii: S2405-8440(23)04147-6
pmc: PMC10361036
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e16940Subventions
Organisme : NIEHS NIH HHS
ID : P30 ES005022
Pays : United States
Organisme : NIEHS NIH HHS
ID : R21 ES032047
Pays : United States
Organisme : NIEHS NIH HHS
ID : T32 ES007148
Pays : United States
Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
We declare that none of the authors have any known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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