Unbiased whole genome detection of ultrarare off-target mutations in genome-edited cell populations by HiFi sequencing.
CRISPR
gene editing
genetic engineering
genotoxicity
next generation sequencing
Journal
Environmental and molecular mutagenesis
ISSN: 1098-2280
Titre abrégé: Environ Mol Mutagen
Pays: United States
ID NLM: 8800109
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
13
07
2023
received:
25
05
2023
accepted:
23
07
2023
medline:
28
8
2023
pubmed:
25
7
2023
entrez:
25
7
2023
Statut:
ppublish
Résumé
DNA base editors (BEs) composed of a nuclease-deficient Cas9 fused to a DNA-modifying enzyme can achieve on-target mutagenesis without creating double-strand DNA breaks (DSBs). As a result, BEs generate far less DNA damage than traditional nuclease-proficient Cas9 systems, which do rely on the creation of DSBs to achieve on-target mutagenesis. The inability of BEs to create DSBs makes the detection of their undesired off-target effects very difficult. PacBio HiFi sequencing can efficiently detect ultrarare mutations resulting from chemical mutagenesis in whole genomes with a sensitivity ~1 × 10
Substances chimiques
RNA, Guide, CRISPR-Cas Systems
0
BES
10191-18-1
DNA
9007-49-2
Types de publication
Journal Article
Research Support, U.S. Gov't, P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
374-381Informations de copyright
Published 2023. This article is a U.S. Government work and is in the public domain in the USA.
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