A new l-serine binding orphan SerBP affects indole synthesis in Pantoea ananatis.
Pantoea ananatis YJ76
amino acid transport
indole
substrate-binding protein
Journal
Journal of basic microbiology
ISSN: 1521-4028
Titre abrégé: J Basic Microbiol
Pays: Germany
ID NLM: 8503885
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
revised:
12
07
2023
received:
29
03
2023
accepted:
15
07
2023
medline:
6
12
2023
pubmed:
27
7
2023
entrez:
26
7
2023
Statut:
ppublish
Résumé
Indole is traditionally known as a metabolite of l-tryptophan and now as an important signaling molecule in bacteria, however, the understanding of its upstream synthesis regulation is very limited. Pantoea ananatis YJ76, a predominant diazotrophic endophyte isolated from rice (Oryza sativa), can produce indole to regulate various physiological and biochemical behaviors. We constructed a mutant library of YJ76 using the mTn5 transposon insertion mutation method, from which an indole-deficient mutant was screened out. Via high-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR), the transposon was determined to be inserted in a gene (RefSeq: WP014605468.1) of unknown function that is highly conserved at the intraspecific level. Bioinformatics analysis implied that the protein (Protein ID: WP089517194.1) encoded by the mutant gene is most likely to be a new orphan substrate-binding protein (SBP) for amino acid ABC transporters. Amino acid supplement cultivation experiments and surface plasmon resonance revealed that the protein could bind to l-serine (K
Identifiants
pubmed: 37495561
doi: 10.1002/jobm.202300165
doi:
Substances chimiques
Amino Acids
0
Indoles
0
Serine
452VLY9402
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1348-1360Subventions
Organisme : National Key Research and Development Program of China
ID : 2018YFA0901800
Organisme : National Natural Science Foundation of China
ID : 31870090
Organisme : Natural Science Foundation of Hebei Province
ID : C2020408006
Informations de copyright
© 2023 Wiley-VCH GmbH.
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