Dynamic full-field optical coherence tomography for live-cell imaging and growth-phase monitoring in
Aspergillus fumigatus
dynamic full-field optical coherence tomography
fungal metabolism
invasive fungal infections
live-cell imaging
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
10
03
2023
accepted:
14
06
2023
medline:
31
7
2023
pubmed:
28
7
2023
entrez:
28
7
2023
Statut:
epublish
Résumé
The diagnosis of cutaneous manifestations of deep mycoses relies on both histopathological and direct examinations. Yet, the current diagnostic criteria cannot prevent missed cases, including invasive aspergillosis, which requires the development of a novel diagnostic approach and imaging tools. We recently introduced the use of dynamic full-field optical coherence tomography (D-FF-OCT) in fungal diagnostics with a definition approaching that of conventional microscopy and the ability to return metabolic information regarding different fungal species. The present work focuses on subcellular dynamics and live-cell imaging of The D-FF-OCT images allow not only the visualization of intracellular trafficking of vacuoles but also an evolving dynamic segmentation of conidiophores depending on the chronological development and aging of the hyphae or the effect of antifungal treatment. The same applies to conidial heads, with the most intense D-FF-OCT signal coming from vesicles, revealing a changing dynamic within a few hours only, as well as complete extinction following subsequent drying of the Sabouraud dextrose agar. These results provide additional data on the ability of D-FF-OCT to monitor some of the main life cycle processes, dynamics, and intracellular trafficking of vacuoles in
Identifiants
pubmed: 37502605
doi: 10.3389/fcimb.2023.1183340
pmc: PMC10369068
doi:
Substances chimiques
Agar
9002-18-0
Antifungal Agents
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1183340Informations de copyright
Copyright © 2023 Maldiney, Garcia-Hermoso, Sitterlé, Chassot, Thouvenin, Boccara, Blot, Piroth, Quenot, Charles, Aimanianda, Podac, Boulnois, Dalle, Sautour, Bougnoux and Lanternier.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Skin Res Technol. 2001 Feb;7(1):1-9
pubmed: 11301634
Biofilm. 2022 Oct 28;4:100090
pubmed: 36389263
mBio. 2020 Feb 4;11(1):
pubmed: 32019790
Lancet Infect Dis. 2017 Dec;17(12):e412-e419
pubmed: 28774694
Med Mycol. 2009;47 Suppl 1:S110-9
pubmed: 19255923
PLoS One. 2022 Dec 30;17(12):e0279825
pubmed: 36584152
Antimicrob Agents Chemother. 2017 Sep 22;61(10):
pubmed: 28760907
J Cancer Res Clin Oncol. 2018 Oct;144(10):1967-1990
pubmed: 29926160
Curr Eye Res. 2015 May;40(5):526-34
pubmed: 25251769
J Am Acad Dermatol. 2019 Apr;80(4):869-880.e5
pubmed: 30102951
Med Mycol. 2009;47 Suppl 1:S72-9
pubmed: 19253141
Sci Rep. 2020 Sep 23;10(1):15519
pubmed: 32968138
Microbiol Mol Biol Rev. 2019 Dec 4;84(1):
pubmed: 31801804
Biomed Opt Express. 2016 Mar 24;7(4):1511-24
pubmed: 27446672
Med Mycol. 2019 Apr 1;57(Supplement_2):S94-S103
pubmed: 30816963
Dermatology. 2012;224(1):84-92
pubmed: 22487768
J Mycol Med. 2022 Nov;32(4):101303
pubmed: 35732095
J Biomed Opt. 2022 May;27(5):
pubmed: 35596250
J Vis Exp. 2017 Dec 24;(130):
pubmed: 29364205
Clin Infect Dis. 2020 Sep 12;71(6):1367-1376
pubmed: 31802125
FEMS Yeast Res. 2018 Aug 1;18(5):
pubmed: 29684195
Light Sci Appl. 2020 Aug 17;9:140
pubmed: 32864115
PLoS One. 2016 Sep 07;11(9):e0162469
pubmed: 27602575
Biotechniques. 2005 Dec;39(6 Suppl):S2-5
pubmed: 20158500
Nat Commun. 2021 Mar 17;12(1):1707
pubmed: 33731708
Nat Commun. 2020 Apr 20;11(1):1868
pubmed: 32313067
PLoS One. 2020 Aug 31;15(8):e0234165
pubmed: 32866179
Mycopathologia. 2006 Aug;162(2):103-9
pubmed: 16897588
Lancet. 2004 Sep 25-Oct 1;364(9440):1173-82
pubmed: 15451228
J Vis Exp. 2019 Nov 28;(153):
pubmed: 31840670
Antimicrob Agents Chemother. 2005 Mar;49(3):945-51
pubmed: 15728887
Acta Ophthalmol. 2022 Mar;100(2):159-163
pubmed: 34031997
Nat Commun. 2018 Dec 3;9(1):5135
pubmed: 30510235
J Vis Exp. 2021 Sep 18;(175):
pubmed: 34605800
Comput Biol Med. 2019 Jan;104:215-219
pubmed: 30529573
J Fungi (Basel). 2022 Dec 08;8(12):
pubmed: 36547618
Nat Methods. 2014 Dec;11(12):1221-8
pubmed: 25423019
J Fungi (Basel). 2022 Dec 26;9(1):
pubmed: 36675860
J Fungi (Basel). 2017 Dec 28;4(1):
pubmed: 29371498
Gut. 2021 Jan;70(1):6-8
pubmed: 32447309
Nat Rev Microbiol. 2017 Nov;15(11):661-674
pubmed: 28919635