Determination of rhein and physcion in rhubarb by microchip capillary electrophoresis in mixed hydro-organic solvent combined with laser-induced fluorescence detection.
Rhei Radixet Rhizoma
laser induced fluorescence
microchip capillary electrophoresis
physcion
rhein
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
06
07
2023
received:
25
03
2023
accepted:
11
07
2023
medline:
9
10
2023
pubmed:
29
7
2023
entrez:
28
7
2023
Statut:
ppublish
Résumé
Microchip capillary electrophoresis in mixed hydro-organic solvent combined with laser-induced fluorescence detection was developed for the separation and detection of physcion and rhein in rhubarb. In contrast to the conventional capillary electrophoresis method, ammonium acetate-dimethyl sulfoxide was used as the basic buffer system in this method. The effects of background buffer, buffer apparent pH*, buffer concentration, water ratio, sample preparation method, and separation voltage on separation and detection were investigated. Optimized separation and detection conditions were obtained: the buffer consisted of 20 mmol/L of ammonium acetate in hydro-organic solvent composed dimethyl sulfoxide, formamide, and water mixed at 60/20/20 (v/v/v) ratio. The separation voltage was 1.9 kV. Under these conditions, the physcion, rhein, and other components of rhubarb can be completely separated within 150 s. Under the methodological verification, good linearity (R ≥ 0.9995) for physcion and rhein, and low limits of detection (0.085 μg·mL
Identifiants
pubmed: 37507831
doi: 10.1002/jssc.202300192
doi:
Substances chimiques
rhein
YM64C2P6UX
physcione
H6PT94IV61
ammonium acetate
RRE756S6Q2
Dimethyl Sulfoxide
YOW8V9698H
Solvents
0
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300192Subventions
Organisme : Department of Science and Technology of Guangdong Province
ID : KTP20210132
Organisme : National Natural Science Foundation of China
ID : 81001600
Informations de copyright
© 2023 Wiley-VCH GmbH.
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