Development of Hydrophobic Cell-Penetrating Stapled Peptides as Drug Carriers.
cell-penetrating peptide
helical structure
hydrophobic peptide
plasmid DNA delivery
stapled peptide
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Jul 2023
21 Jul 2023
Historique:
received:
22
06
2023
revised:
07
07
2023
accepted:
19
07
2023
medline:
31
7
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
epublish
Résumé
Cell-penetrating peptides (CPPs) are widely used for the intracellular delivery of a variety of cargo molecules, including small molecules, peptides, nucleic acids, and proteins. Many cationic and amphiphilic CPPs have been developed; however, there have been few reports regarding hydrophobic CPPs. Herein, we have developed stapled hydrophobic CPPs based on the hydrophobic CPP, TP10, by introducing an aliphatic carbon side chain on the hydrophobic face of TP10. This side chain maintained the hydrophobicity of TP10 and enhanced the helicity and cell penetrating efficiency. We evaluated the preferred secondary structures, and the ability to deliver 5(6)-carboxyfluorescein (CF) as a model small molecule and plasmid DNA (pDNA) as a model nucleotide. The stapled peptide
Identifiants
pubmed: 37511527
pii: ijms241411768
doi: 10.3390/ijms241411768
pmc: PMC10380766
pii:
doi:
Substances chimiques
Drug Carriers
0
Cell-Penetrating Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Agency for Medical Research and Development
ID : 23mk0101197 (to Y.D.), 23ae0121013 (to Y.D.), 23ak0101185 (to Y.D.), 23mk0101220 (to Y.D.), 23fk0210110 (to Y.D.), and 23fk0310506 (to Y.D.)
Organisme : Japan Society for the Promotion of Science
ID : JP21K05320 to Y.D.; JP23H04926 to Y.D.; JP22K15257 to H.Y.
Organisme : JST ACT-X
ID : JPMJAX222L, Japan to H.Y.
Organisme : Takeda Science Foundation
ID : (to H.Y.)
Références
Mol Pharm. 2014 Mar 3;11(3):964-73
pubmed: 24437690
J Control Release. 2016 May 10;229:130-139
pubmed: 26993425
ACS Nano. 2014 Mar 25;8(3):1972-94
pubmed: 24559246
Bioconjug Chem. 2001 Nov-Dec;12(6):1005-11
pubmed: 11716693
Biochim Biophys Acta. 2000 Jul 31;1467(1):165-76
pubmed: 10930519
J Am Chem Soc. 2017 Nov 8;139(44):15628-15631
pubmed: 28992407
Chem Rev. 2019 Sep 11;119(17):9915-9949
pubmed: 31045350
Biol Pharm Bull. 2012;35(7):1064-8
pubmed: 22791153
Acc Chem Res. 2012 Jul 17;45(7):1132-9
pubmed: 22208383
Pharmacol Rev. 2006 Mar;58(1):32-45
pubmed: 16507881
Chembiochem. 2016 Jan;17(2):137-40
pubmed: 26560998
Biochim Biophys Acta. 2012 Mar;1818(3):502-11
pubmed: 22155257
Biomed Rep. 2016 May;4(5):528-534
pubmed: 27123243
FASEB J. 1998 Jan;12(1):67-77
pubmed: 9438412
Chemistry. 2000 Dec 15;6(24):4498-504
pubmed: 11192082
J Biophys. 2011;2011:414729
pubmed: 21687343
Acc Chem Res. 2012 Jul 17;45(7):1048-56
pubmed: 22455499
ACS Appl Bio Mater. 2021 Aug 16;4(8):6404-6416
pubmed: 35006917
FASEB J. 2001 Jun;15(8):1451-3
pubmed: 11387254
Front Bioeng Biotechnol. 2020 Mar 04;8:162
pubmed: 32195241